Evidence map›Paper›PMID 39464079›Full record

ArticlebioRxiv : the preprint server for biology2024

Single-Cell Antigen Receptor Sequencing in Pigs with Influenza.

Weihong Gu, Darling Melany de Carvahlo Madrid, Sadie Clements, Laurie Touchard, Nathan Bivins, Grant Zane, Mingyi Zhou, Kiho Lee, John P Driver

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Weihong GuDivision of Animal Sciences, University of Missouri, Columbia, MO, USA.
Darling Melany de Carvahlo MadridDivision of Animal Sciences, University of Missouri, Columbia, MO, USA.
Sadie ClementsDivision of Animal Sciences, University of Missouri, Columbia, MO, USA.
Laurie TouchardDivision of Animal Sciences, University of Missouri, Columbia, MO, USA.
Nathan BivinsGenomics Technology Core, University of Missouri, Columbia, MO, USA.
Grant ZaneGenomics Technology Core, University of Missouri, Columbia, MO, USA.
Mingyi ZhouGenomics Technology Core, University of Missouri, Columbia, MO, USA.
Kiho LeeDivision of Animal Sciences, University of Missouri, Columbia, MO, USA.
John P DriverDivision of Animal Sciences, University of Missouri, Columbia, MO, USA.

Funding

Genetically modified pigs to model NKT cell immunity to influenza virus infectionR01AI158477 · NIAID · UNIVERSITY OF MISSOURI-COLUMBIA · PI John Driver, Juergen A Richt · 2022 to 2026
$3.2M
Harnessing Natural Killer T cells to counteract swine influenzaR01HD092286 · NICHD · UNIVERSITY OF MISSOURI-COLUMBIA · PI DRIVER, JOHN · 2017 to 2021
$2.6M
NIAID NIH HHS R01 AI158477NICHD NIH HHS R01 HD092286
6 · The paper itself

Abstract

Understanding the pulmonary adaptive immune system of pigs is important as respiratory pathogens present a major challenge for swine producers and pigs are increasingly used to model human pulmonary diseases. Single-cell RNA sequencing (scRNAseq) has accelerated the characterization of cellular phenotypes in the pig respiratory tract under both healthy and diseased conditions. However, combining scRNAseq with recovery of paired T cell receptor (TCR) α and β chains as well as B cell receptor (BCR) heavy and light chains to interrogate their repertoires has not to our knowledge been demonstrated for pigs. Here, we developed primers to enrich porcine TCR α and β chains along with BCR κ and λ light chains and IgM, IgA, and IgG heavy chains that are compatible with the 10x Genomics VDJ sequencing protocol. Using these pig-specific assays, we sequenced the T and B cell receptors of cryopreserved lung cells from

Indexed as

B cell receptorgenetically edited swineinfluenza A virus natural killer T cellslung cellsPigssingle cell RNA sequencingT cell receptor

Identifiers

PMID39464079
PMCPMC11507742

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.