Evidence map›Paper›PMID 39462945›Full record

ArticlePlant, cell & environment2025

Hyperspectral Imaging Reveals Differential Carotenoid and Chlorophyll Temporal Dynamics and Spatial Patterns in Scots Pine Under Water Stress.

Iiro Miettinen, Chao Zhang, Luis Alonso, Beatriz Fernández-Marín, José I García-Plazaola, Steffen Grebe, Albert Porcar-Castell, Jon Atherton

Abstract read
In one paragraph

Article in Plant, cell & environment, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Colored Leaves ofACS omega · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Iiro MiettinenOptics of Photosynthesis Laboratory, Department of Forest Sciences, Institute for Atmospheric and Earth System Research (INAR), Faculty of Agriculture and Forestry, University of Helsinki, Helsinki, Uusimaa, Finland.ORCID https://orcid.org/0009-0008-6171-0942
Chao ZhangOptics of Photosynthesis Laboratory, Department of Forest Sciences, Institute for Atmospheric and Earth System Research (INAR), Faculty of Agriculture and Forestry, University of Helsinki, Helsinki, Uusimaa, Finland.ORCID http://orcid.org/0000-0001-7327-9477
Luis AlonsoOptics of Photosynthesis Laboratory, Department of Forest Sciences, Institute for Atmospheric and Earth System Research (INAR), Faculty of Agriculture and Forestry, University of Helsinki, Helsinki, Uusimaa, Finland.ORCID https://orcid.org/0000-0002-8221-5739
Beatriz Fernández-MarínDepartment of Plant Biology and Ecology, University of the Basque Country (UPV/EHU), Leioa, Basque Country, Spain.ORCID https://orcid.org/0000-0001-9951-0489
José I García-PlazaolaDepartment of Plant Biology and Ecology, University of the Basque Country (UPV/EHU), Leioa, Basque Country, Spain.ORCID https://orcid.org/0000-0001-6498-975X
Steffen GrebeOptics of Photosynthesis Laboratory, Department of Forest Sciences, Institute for Atmospheric and Earth System Research (INAR), Faculty of Agriculture and Forestry, University of Helsinki, Helsinki, Uusimaa, Finland.ORCID https://orcid.org/0000-0001-9718-1570
Albert Porcar-CastellOptics of Photosynthesis Laboratory, Department of Forest Sciences, Institute for Atmospheric and Earth System Research (INAR), Faculty of Agriculture and Forestry, University of Helsinki, Helsinki, Uusimaa, Finland.ORCID https://orcid.org/0000-0003-1357-9982
Jon AthertonOptics of Photosynthesis Laboratory, Department of Forest Sciences, Institute for Atmospheric and Earth System Research (INAR), Faculty of Agriculture and Forestry, University of Helsinki, Helsinki, Uusimaa, Finland.ORCID http://orcid.org/0000-0002-7670-2928

Funding

Jon Atherton was funded by the Research Council of Finland (347929, 353804). Chao Zhang was funded by the Research Council of Finland (340744). Beatriz Fernández-Marín and José I. García-Plazaola were funded by the Basque Government, Spain (grant UPV/EHU-GV IT-1648-22).
6 · The paper itself

Abstract

Drought-related die-off events have been observed throughout Europe in Scots pine (Pinus sylvestris L.). Such events are exacerbated by carbon starvation that is, an imbalance of photosynthetic productivity and resource usage. Recent evidence suggests that optically measurable photosynthetic pigments such as chlorophylls and carotenoids respond to water stress (WS). However, there is a lack of measurements using imaging spectroscopy, and the mechanisms linking xanthophyll-related changes in reflectance captured by the photochemical reflectance index (PRI) and chlorophyll changes in red edge position (REP) to WS are not understood. To probe this, we conducted a greenhouse experiment where 3-year-old Pinus sylvestris saplings were subjected to water limitation and followed using hyperspectral imaging (HSI) spectroscopy, water status and photosynthetic measurements. Carotenoids (e.g., xanthophyll cycle) and chlorophylls responded to WS, which was observed using the HSI-derived indices PRI and REP respectively. The spatial-temporal response in these two pigment-reflectance groupings differed. The spatial distribution of PRI represented the light intensity around the time of the measurement, whereas REP reflected the daily averaged light intensity over the experimental course. A further difference was noted upon rewatering, where the carotenoid-related PRI partially recovered but the chlorophyll-related REP did not.

Indexed as

CarotenoidsChlorophyllPinus sylvestrisDehydrationDroughtsHyperspectral ImagingPhotosynthesisPlant LeavesWaterCarotenoidsChlorophyllWaterdroughthyperspectral imagingphotochemical reflectance indexred edgeremote sensingScots pinewater stressxanthophyll cycle

Identifiers

PMID39462945
PMCPMC11695750

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.