Interaction of RECQL4 with poly(ADP-ribose) is critical for the DNA double-strand break response in human cells.

Sunyoung Shin, Dongmin Kim, Hyemi Kim, Won-Ho Cho, Gyungmin Kim, Joon-Kyu Lee

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Article in FEBS open bio, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

6 authors.

Sunyoung ShinDepartment of Biology Education, Seoul National University, Korea.

Dongmin KimDepartment of Biology Education, Seoul National University, Korea.

Hyemi KimDepartment of Biology Education, Seoul National University, Korea.

Won-Ho ChoDepartment of Biology Education, Seoul National University, Korea.

Gyungmin KimDepartment of Biology Education, Seoul National University, Korea.

Joon-Kyu LeeDepartment of Biology Education, Seoul National University, Korea.ORCID https://orcid.org/0000-0003-4485-5812

Funding

National Research Foundation of Korea NRF-2020R1H1A2101822National Research Foundation of Korea NRF-2021R1F1A1048993

6 · The paper itself

Abstract

To overcome genotoxicity, cells have evolved powerful and effective mechanisms to detect and respond to DNA lesions. RecQ Like Helicase-4 (RECQL4) plays a vital role in DNA damage responses. RECQL4 is recruited to DNA double-strand break (DSB) sites in a poly(ADP-ribosyl)ation (PARylation)-dependent manner, but the mechanism and significance of this process remain unclear. Here, we showed that the domain of RECQL4 recruited to DSBs in a PARylation-dependent manner directly interacts with poly(ADP-ribose) (PAR) and contains a PAR-binding motif (PBM). By replacing this PBM with a PBM of hnRNPA2 or its mutated form, we demonstrated that the PBM in RECQL4 is required for PARylation-dependent recruitment and the roles of RECQL4 in the DSB response. These results suggest that the direct interaction of RECQL4 with PAR is critical for proper cellular response to DSBs and provide insights to understand PARylation-dependent control of the DSB response and cancer therapeutics using PARylation inhibitors.

Indexed as

DNA Breaks, Double-StrandedPoly Adenosine Diphosphate RiboseRecQ HelicasesDNA RepairHumansProtein BindingPoly Adenosine Diphosphate RiboseRecQ HelicasesRECQL4 protein, humanDNA double‐strand breaksDNA repairpoly(ADP‐ribose) binding motifpoly(ADP‐ribosyl)ationRECQL4

Identifiers

PMID39462683

DOI10.1002/2211-5463.13917

PMCPMC11705483

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.