Evidence map›Paper›PMID 39462108›Full record

ArticleScientific reports2024

Dibenzolium induces apoptosis and inhibits epithelial-mesenchymal transition (EMT) in bladder cancer cell lines.

Yura Jotatsu, Jack L Arbiser, Michika Moriwaki, Yuto Hirata, Shunya Takeda, Ichiro Takada, Kuan-Chou Chen, Shian-Ying Sung, Katsumi Shigemura

Abstract read
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In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yura JotatsuDepartment of International Health, Kobe University Graduate School of Health Sciences, 7-10-2 Tomogaoka, Sumaku, Kobe, 654-0142, Japan.
Jack L ArbiserMetroderm United Derm Partners, Atlanta, GA, USA.
Michika MoriwakiDepartment of International Health, Kobe University Graduate School of Health Sciences, 7-10-2 Tomogaoka, Sumaku, Kobe, 654-0142, Japan.
Yuto HirataDepartment of International Health, Kobe University Graduate School of Health Sciences, 7-10-2 Tomogaoka, Sumaku, Kobe, 654-0142, Japan.
Shunya TakedaDepartment of Medical Device Engineering, Kobe University Graduate School of Medicine, 7-5-1 Kusunokicho, Kobe, 650-0017, Japan.
Ichiro TakadaDepartment of Urology, Teikyo University Hospital, Teikyo University Graduate School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo, 173-8605, Japan.
Kuan-Chou ChenDepartment of Urology, Taipei Medical University Shuang Ho Hospital, 291, Zhongzheng Road, Taipei, 235, Taiwan.
Shian-Ying SungThe Ph.D. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, 250 Wu-Hsing st., Taipei, 110, Taiwan.
Katsumi ShigemuraDepartment of Medical Device Engineering, Kobe University Graduate School of Medicine, 7-5-1 Kusunokicho, Kobe, 650-0017, Japan. shigemura.katsumi.up@teikyo-u.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bladder cancer treatments are highly aggressive and have strong side effects. Safer and more effective treatments are needed. In this study, Dibenzolium (DIB), a potent NADPH oxidase inhibitor, was evaluated for its anti-tumor effects. KK-47 (non-invasive), T24 and 5637 (invasive) cells were used in experiments. Cell proliferation, apoptosis and wound healing assays and western blotting were conducted. In addition, DIB was intratumorally administered to mice bearing KK-47, T24 and 5637 tumors, and tumor size and weight were observed over time. After removing tumors, immunohistochemistry (IHC) staining was conducted. Cell proliferation was significantly suppressed in all cell lines, and apoptotic cells increased in the KK-47 and T24 cell lines after DIB. Wound healing was suppressed in all cell lines by DIB. In KK-47 and T24, DIB increased the protein expression of the epithelial marker E-cadherin. In vivo, DIB safely suppressed tumor growth in all cell lines-bearing mice. Cleaved-Caspase-3 and E-cadherin expression increased in KK-47 and T24 tumors after DIB. In conclusion, DIB inhibited tumor growth by inducing apoptosis through the Caspase-3 pathway and reduced migration and invasion by suppressing epithelial mesenchymal transition (EMT) in bladder cancer similarly shown as our previous study of prostate cancer.

Indexed as

ApoptosisCell ProliferationEpithelial-Mesenchymal TransitionUrinary Bladder NeoplasmsAnimalsAntineoplastic AgentsCell Line, TumorCell MovementHumansMiceMice, NudeXenograft Model Antitumor AssaysAntineoplastic AgentsApoptosisBladder cancerDibenzoliumEpithelial mesenchymal transition

Identifiers

PMID39462108
PMCPMC11513011

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.