Evidence map›Paper›PMID 39460911›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Large-Scale Transient Transfection of Suspension-Adapted Chinese Hamster Ovary Cells for the Production of the Trimeric SARS-CoV-2 Spike Protein.

David L Hacker, Kelvin Lau, Laurence Durrer, Soraya Quinche, Michael François, Ludovic Delisle, Florence Pojer

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

David L HackerProtein Production and Structure Core Facility (PTPSP), École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
Kelvin LauProtein Production and Structure Core Facility (PTPSP), École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland. kelvin.lau@epfl.ch.
Laurence DurrerProtein Production and Structure Core Facility (PTPSP), École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
Soraya QuincheProtein Production and Structure Core Facility (PTPSP), École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
Michael FrançoisProtein Production and Structure Core Facility (PTPSP), École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
Ludovic DelisleProtein Production and Structure Core Facility (PTPSP), École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
Florence PojerProtein Production and Structure Core Facility (PTPSP), École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland. florence.pojer@epfl.ch.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The production and purification of the secreted ectodomain of SARS-CoV-2 spike protein (S protein) were performed by transiently transfecting suspension-adapted Chinese hamster ovary cells (ExpiCHO). The method involved the separate addition of plasmid DNA expressing the S protein and polyethyleneimine to a suspension culture at a density of 5 × 10

Indexed as

CricetulusSARS-CoV-2Spike Glycoprotein, CoronavirusTransfectionAnimalsCHO CellsCricetinaePlasmidsRecombinant ProteinsRecombinant ProteinsSpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2CHO cellsOrbital shakingPolyethyleneimineProtein purificationRecombinant proteinTransfection

Identifiers

PMID39460911

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.