Evidence map›Paper›PMID 39460771›Full record

ArticleAnalytical and bioanalytical chemistry2024

Preparation of divalent camelid single-domain antibody and its application in immunoassays for Salmonella detection in food.

Yanhong Chen, Yingjie Huang, Ruo Yang, Yongliang Cui, Yanru Wang, Xiaohong Wang, Jia Wang

Abstract read
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Article in Analytical and bioanalytical chemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. An immunoassay based on polyHRP signal amplification for HPV16/18 E6 RNA detection with anti-DNA-RNA hybrid antibody.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yanhong ChenCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Yingjie HuangCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Ruo YangCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Yongliang CuiLaboratory of Quality & Safety Risk Assessment for Citrus Products (Chongqing), Ministry of Agriculture, Citrus Research Institute, Southwest University, Chongqing, 400712, China.
Yanru WangCollege of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Xiaohong WangCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Jia WangCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China. wangjia@mail.hzau.edu.cn.ORCID http://orcid.org/0000-0001-6036-1201

Funding

National Innovation and Entrepreneurship Training Program for Undergraduate 202210504076National Natural Science Foundation of China 32272438
6 · The paper itself

Abstract

Salmonella-related foodborne infections are commonly caused by the serovars of S. Typhimurium, which can be detected using antibody-based immunoassays. The monovalent variable domain of the camelid heavy chain antibody (VHH) performs excellently in constructing multivalent VHH variants, which generally exhibit higher affinities with antigens and consequently enhance the assay sensitivity. In this study, the divalent variants of VHHs (diVHHs) targeting S. Typhimurium were generated by encoding the monovalent VHH genes assembled in tandem with a flexible linker peptide (G

Indexed as

Food MicrobiologyLimit of DetectionSingle-Domain AntibodiesAnimalsCamelidaeChickensFood ContaminationImmunoassayLactucaSalmonellaSalmonella typhimuriumSwineSingle-Domain AntibodiesDivalent VHHsFoodSalmonellaSandwich ELISAVHH antibody

Identifiers

PMID39460771

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.