Evidence map›Paper›PMID 39459902›Full record

ArticleViruses2024

Hepatitis Delta Virus Clade 8 Is the Predominant Clade Circulating in Botswana amongst People Living with HIV.

Kabo Baruti, Wonderful T Choga, Patience C Motshosi, Bonolo B Phinius, Basetsana Phakedi, Lynnette N Bhebhe, Gorata G A Mpebe, Chanana D Tsayang, Tsholofelo Ratsoma, Tendani Gaolathe and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Kabo BarutiResearch Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.
Wonderful T ChogaResearch Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.ORCID 0000-0001-7606-0569
Patience C MotshosiResearch Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.
Bonolo B PhiniusResearch Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.ORCID 0000-0002-0180-2705
Basetsana PhakediResearch Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.
Lynnette N BhebheResearch Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.ORCID 0000-0003-0282-0793
Gorata G A MpebeResearch Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.
Chanana D TsayangResearch Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.
Tsholofelo RatsomaResearch Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.
Tendani GaolatheResearch Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.
Mosepele MosepeleResearch Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.
Joseph MakhemaResearch Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.
Roger ShapiroResearch Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.
Shahin LockmanResearch Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.
Sikhulile MoyoResearch Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.ORCID 0000-0003-3821-4592
Mosimanegape JongmanResearch Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.
Motswedi AndersonResearch Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.ORCID 0000-0001-9974-9684
Simani GaseitsiweResearch Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.

Funding

Training in HIV Genomics, Treatment, and Cure Research in BotswanaD43TW009610 · FIC · THE BOTSWANA HARVARD HEALTH PARTNERSHIP · PI SHAHIN LOCKMAN, Joseph Moeketsi Makhema · 2013 to 2026
$4.4M
Viral Diversity an Innovative Biomarker for Refining Estimates of HIV IncidenceK43TW012350 · FIC · THE BOTSWANA HARVARD HEALTH PARTNERSHIP · PI Sikhulile Moyo · 2022 to 2026
$682k
CGH CDC HHS U01 GH000447CGH CDC HHS U2G GH001911FIC NIH HHS D43 TW009610FIC NIH HHS K43 TW012350Wellcome Trust 218770/Z/19/ZWellcome Trust 224802/Z/21/Z
6 · The paper itself

Abstract

Hepatitis delta virus (HDV) co-infections more often result in severe hepatitis compared to hepatitis B virus (HBV) infections alone. Despite a high HDV prevalence (7.1%), information regarding circulating HDV clades is very limited in Botswana. We extracted total nucleic acid from confirmed HDV-positive samples and quantified their viral load. We then sequenced the large hepatitis delta antigen (L-HDAg) using Oxford Nanopore Technology (ONT). Genotyping was performed using the HDV Database, and HDV mutation profiling was performed on AliView. All participants with HBV genotypic information belonged to sub-genotype A1, and 80% (4/5) of them had a higher HDV viral load and a lower HBV viral load. We sequenced 75% (9/12) of the HDV-positive samples, which belonged to HDV clade 8. A total of 54 mutations were discovered, with the most prevalent being Q148R (16%), D149P (16%) and G151D (16%). Known mutations such as S117A, K131R, R139K and G151D were detected, while the other mutations were novel. Our results reveal that HDV clade 8 is the predominant clade in Botswana. The significance of all mutations remains unclear. Future studies with a larger sample size to detect other HDV clades that might be circulating in Botswana and functionally characterize the detected mutations are warranted.

Indexed as

CoinfectionGenotypeHepatitis DHepatitis Delta VirusHIV InfectionsMutationPhylogenyViral LoadAdultBotswanaFemaleHepatitis BHepatitis B virusHumansMaleMiddle AgedBotswanacladeshepatitis delta virusmutations

Identifiers

PMID39459902
PMCPMC11512356

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.