Evidence map›Paper›PMID 39459891›Full record

ArticleViruses2024

Discriminating North American Swine Influenza Viruses with a Portable, One-Step, Triplex Real-Time RT-PCR Assay, and Portable Sequencing.

Marie K Kirby, Bo Shu, Matthew W Keller, Malania M Wilson, Benjamin L Rambo-Martin, Yunho Jang, Jimma Liddell, Eduardo Salinas Duron, Jacqueline M Nolting, Andrew S Bowman and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Marie K KirbyVirology, Surveillance and Diagnostic Branch, Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.ORCID 0000-0003-0859-9604
Bo ShuVirology, Surveillance and Diagnostic Branch, Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.
Matthew W KellerVirology, Surveillance and Diagnostic Branch, Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.ORCID 0000-0002-5850-1698
Malania M WilsonVirology, Surveillance and Diagnostic Branch, Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.ORCID 0000-0003-3486-1725
Benjamin L Rambo-MartinVirology, Surveillance and Diagnostic Branch, Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.
Yunho JangVirology, Surveillance and Diagnostic Branch, Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.
Jimma LiddellVirology, Surveillance and Diagnostic Branch, Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.
Eduardo Salinas DuronVirology, Surveillance and Diagnostic Branch, Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.ORCID 0000-0002-4490-9289
Jacqueline M NoltingDepartment of Veterinary Preventive Medicine, The Ohio State University, Columbus, OH 43210, USA.ORCID 0000-0002-2705-4805
Andrew S BowmanDepartment of Veterinary Preventive Medicine, The Ohio State University, Columbus, OH 43210, USA.ORCID 0000-0002-0738-8453
C Todd DavisVirology, Surveillance and Diagnostic Branch, Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.
David E WentworthVirology, Surveillance and Diagnostic Branch, Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.ORCID 0000-0002-5190-980X
John R BarnesVirology, Surveillance and Diagnostic Branch, Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Swine harbors a genetically diverse population of swine influenza A viruses (IAV-S), with demonstrated potential to transmit to the human population, causing outbreaks and pandemics. Here, we describe the development of a one-step, triplex real-time reverse transcription-polymerase chain reaction (rRT-PCR) assay that detects and distinguishes the majority of the antigenically distinct influenza A virus hemagglutinin (HA) clades currently circulating in North American swine, including the IAV-S H1 1A.1 (α), 1A.2 (β), 1A.3 (γ), 1B.2.2 (δ1) and 1B.2.1 (δ2) clades, and the IAV-S H3 2010.1 clade. We performed an in-field test at an exhibition swine show using in-field viral concentration and RNA extraction methodologies and a portable real-time PCR instrument, and rapidly identified three distinct IAV-S clades circulating within the N.A. swine population. Portable sequencing is used to further confirm the results of the in-field test of the swine triplex assay. The IAV-S triplex rRT-PCR assay can be easily transported and used in-field to characterize circulating IAV-S clades in North America, allowing for surveillance and early detection of North American IAV-S with human outbreak and pandemic potential.

Indexed as

Influenza A virusOrthomyxoviridae InfectionsSwine DiseasesAnimalsHemagglutinin Glycoproteins, Influenza VirusMultiplex Polymerase Chain ReactionNorth AmericaPhylogenyReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionRNA, ViralSensitivity and SpecificitySwineHemagglutinin Glycoproteins, Influenza VirusRNA, Viralportable sequencingswine influenza virustriplex real-time RT-PCR

Identifiers

PMID39459891
PMCPMC11512246

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.