Modelling a Western Lifestyle in Mice: A Novel Approach to Eradicating Aerobic Spore-Forming Bacteria from the Colonic Microbiome and Assessing Long-Term Clinical Outcomes.

Edward Horwell, William Ferreira, Huynh Hong, Philip Bearn, Simon Cutting

Abstract read

In one paragraph

Article in Biomedicines, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Edward HorwellColorectal Surgical Unit, Ashford & St Peter's Hospitals NHS Foundation Trust, London KT16 0PZ, UK.

William FerreiraBiomedical Science Unit, Royal Holloway University of London, London TW20 0EY, UK.

Huynh HongBiomedical Science Unit, Royal Holloway University of London, London TW20 0EY, UK.

Philip BearnColorectal Surgical Unit, Ashford & St Peter's Hospitals NHS Foundation Trust, London KT16 0PZ, UK.

Simon CuttingBiomedical Science Unit, Royal Holloway University of London, London TW20 0EY, UK.

Funding

Medical Research Council MR/R026262/1

6 · The paper itself

Abstract

introductionThe environmentally acquired aerobic spore-forming (EAS-Fs) bacteria that are ubiquitous in nature (e.g., soil) are transient colonisers of the mammalian gastro-intestinal tract. Without regular exposure, their numbers quickly diminish. These species of bacteria have been suggested to be essential to the normal functioning of metabolic and immunogenic health. The modern Western lifestyle restricts exposure to these EAS-Fs, possibly explaining part of the pathogenesis of many Western diseases. To date, the only animal studies that address specific microbiome modelling are based around germ-free animals. We have designed a new animal model that specifically restricts exposure to environmental sources of bacteria. METHODOLOGY: A new protocol, termed

resultsOur results show that stringent adherence to the

conclusionsThis new murine model successfully isolates EAS-Fs and has potential utility for future research, allowing for an investigation into the clinical impact of living in relative hygienic conditions.

Indexed as

animal modelsbacillusgastrointestinal microbiomemetabolismold friends hypothesisspore-forming bacteria

Identifiers

PMID39457587

DOI10.3390/biomedicines12102274

PMCPMC11504893

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.