Evidence map›Paper›PMID 39456881›Full record

ArticleInternational journal of molecular sciences2024

Development of Malate Biosensor-Containing Hydrogels and Living Cell-Based Sensors.

Nathan J Ricks, Monica Brachi, Kevin McFadden, Rohit G Jadhav, Shelley D Minteer, Ming C Hammond

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Nathan J RicksDepartment of Chemistry, University of Utah, Salt Lake City, UT 84112, USA.
Monica BrachiKummer Institute Center for Resource Sustainability, Missouri University of Science and Technology, Rolla, MO 65409, USA.ORCID 0000-0003-1749-648X
Kevin McFaddenDepartment of Chemistry, University of Utah, Salt Lake City, UT 84112, USA.
Rohit G JadhavKummer Institute Center for Resource Sustainability, Missouri University of Science and Technology, Rolla, MO 65409, USA.
Shelley D MinteerDepartment of Chemistry, University of Utah, Salt Lake City, UT 84112, USA.
Ming C HammondDepartment of Chemistry, University of Utah, Salt Lake City, UT 84112, USA.

Funding

Office of Naval Research N000142112188
6 · The paper itself

Abstract

Malate is a key intermediate in the citric acid cycle, an enzymatic cascade that is central to cellular energy metabolism and that has been applied to make biofuel cells. To enable real-time sensing of malate levels, we have engineered a genetically encoded, protein-based fluorescent biosensor called Malon specifically responsive to malate by performing structure-based mutagenesis of the Cache-binding domain of the Citron GFP-based biosensor. Malon demonstrates high specificity and fluorescence activation in response to malate, and has been applied to monitor enzymatic reactions in vitro. Furthermore, we successfully incorporated Malon into redox polymer hydrogels and bacterial cells, enabling analysis of malate levels in these materials and living systems. These results show the potential for fluorescent biosensors in enzymatic cascade monitoring within biomaterials and present Malon as a novel tool for bioelectronic devices.

Indexed as

Biosensing TechniquesHydrogelsMalatesEscherichia coliGreen Fluorescent ProteinsGreen Fluorescent ProteinsHydrogelsMalatesmalic acidbioelectrodeCachecell-based biosensorDcuS

Identifiers

PMID39456881
PMCPMC11507523

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.