Evidence map›Paper›PMID 39456241›Full record

ArticleBiomolecules2024

Expression of Myeloperoxidase in Patient-Derived Endothelial Colony-Forming Cells-Associations with Coronary Artery Disease and Mitochondrial Function.

Weiqian Eugene Lee, Elijah Genetzakis, Giannie Barsha, Joshua Vescovi, Carmen Mifsud, Stephen T Vernon, Tung Viet Nguyen, Michael P Gray, Stuart M Grieve, Gemma A Figtree

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In one paragraph

Article in Biomolecules, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Weiqian Eugene LeeKolling Institute, 10 Westbourne Street, St Leonards, Sydney, NSW 2064, Australia.ORCID 0000-0002-2232-1759
Elijah GenetzakisKolling Institute, 10 Westbourne Street, St Leonards, Sydney, NSW 2064, Australia.
Giannie BarshaThe Victorian Heart Institute and Biomedicine Discovery Institute, Monash University, Wellington Road, Clayton, Melbourne, VIC 3800, Australia.
Joshua VescoviKolling Institute, 10 Westbourne Street, St Leonards, Sydney, NSW 2064, Australia.ORCID 0009-0007-0845-6204
Carmen MifsudKolling Institute, 10 Westbourne Street, St Leonards, Sydney, NSW 2064, Australia.ORCID 0009-0001-7932-5400
Stephen T VernonKolling Institute, 10 Westbourne Street, St Leonards, Sydney, NSW 2064, Australia.
Tung Viet NguyenKolling Institute, 10 Westbourne Street, St Leonards, Sydney, NSW 2064, Australia.ORCID 0000-0003-1294-7739
Michael P GrayKolling Institute, 10 Westbourne Street, St Leonards, Sydney, NSW 2064, Australia.ORCID 0000-0003-1154-3011
Stuart M GrieveCharles Perkins Centre, Johns Hopkins Drive, Camperdown, Sydney, NSW 2050, Australia.ORCID 0000-0002-2712-157X
Gemma A FigtreeKolling Institute, 10 Westbourne Street, St Leonards, Sydney, NSW 2064, Australia.

Funding

National Health and Medical Research Council Australia GNT2018194, GNT2005791NSW Office of Health and Medical Research H21/174585
6 · The paper itself

Abstract

BACKGROUND AND

aimsMyeloperoxidase (MPO) plays a critical role in the innate immune response and has been suggested to be a surrogate marker of oxidative stress and inflammation, with elevated levels implicated in cardiovascular diseases, such as atherosclerosis and heart failure, as well as in conditions like rheumatoid arthritis and cancer. While MPO is well-known in leukocytes, its expression and function in human endothelial cells remain unclear. This study investigates MPO expression in patient-derived endothelial colony-forming cells (ECFCs) and its potential association with CAD and mitochondrial function.

methodsECFCs were cultured from the peripheral blood of 93 BioHEART-CT patients. MPO expression and associated functions were examined using qRT-PCR, immunochemistry, flow cytometry, and MPO activity assays. CAD presence was defined using CT coronary angiography (CACS > 0).

resultsWe report MPO presence in patient-derived ECFCs for the first time. MPO protein expression occurred in 70.7% of samples (

conclusionsMPO is present in the nucleus of ECFCs. In non-CAD ECFCs, MPO expression is linked to upregulated mitochondrial-antioxidant genes, whereas in CAD ECFCs, it is associated with greater mitochondrial dysfunction.

Indexed as

Coronary Artery DiseaseMitochondriaPeroxidaseAgedCells, CulturedEndothelial CellsEndothelial Progenitor CellsFemaleHumansMaleMiddle AgedMPO protein, humanPeroxidasecoronary artery diseasediscoveryendothelial colony forming cellsendothelial dysfunctionmyeloperoxidase

Identifiers

PMID39456241
PMCPMC11505856

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.