Evidence map›Paper›PMID 39456226›Full record

ArticleBiomolecules2024

The Formation of Stable Lung Tumor Spheroids during Random Positioning Involves Increased Estrogen Sensitivity.

Balkis Barkia, Viviann Sandt, Daniela Melnik, José Luis Cortés-Sánchez, Shannon Marchal, Bjorn Baselet, Sarah Baatout, Jayashree Sahana, Daniela Grimm, Markus Wehland and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomolecules, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Balkis BarkiaDepartment of Microgravity and Translational Regenerative Medicine, Otto von Guericke University, 39106 Magdeburg, Germany.
Viviann SandtDepartment of Microgravity and Translational Regenerative Medicine, Otto von Guericke University, 39106 Magdeburg, Germany.
Daniela MelnikDepartment of Microgravity and Translational Regenerative Medicine, Otto von Guericke University, 39106 Magdeburg, Germany.
José Luis Cortés-SánchezDepartment of Microgravity and Translational Regenerative Medicine, Otto von Guericke University, 39106 Magdeburg, Germany.ORCID 0000-0002-4646-9520
Shannon MarchalDepartment of Microgravity and Translational Regenerative Medicine, Otto von Guericke University, 39106 Magdeburg, Germany.ORCID 0000-0002-5987-2159
Bjorn BaseletRadiobiology Unit, Belgian Nuclear Research Centre SCK-CEN, 2400 Mol, Belgium.ORCID 0000-0001-9840-2822
Sarah BaatoutRadiobiology Unit, Belgian Nuclear Research Centre SCK-CEN, 2400 Mol, Belgium.ORCID 0000-0001-8110-751X
Jayashree SahanaDepartment of Biomedicine, Faculty of Health, Aarhus University, 8000 Aarhus, Denmark.
Daniela GrimmDepartment of Biomedicine, Faculty of Health, Aarhus University, 8000 Aarhus, Denmark.ORCID 0000-0002-4991-3105
Markus WehlandDepartment of Microgravity and Translational Regenerative Medicine, Otto von Guericke University, 39106 Magdeburg, Germany.ORCID 0000-0002-8160-859X
Herbert SchulzDepartment of Microgravity and Translational Regenerative Medicine, Otto von Guericke University, 39106 Magdeburg, Germany.ORCID 0000-0002-0226-964X
Manfred InfangerResearch Group "Magdeburger Arbeitsgemeinschaft für Forschung unter Raumfahrt- und Schwerelosigkeitsbedingungen" (MARS), Otto von Guericke University, 39106 Magdeburg, Germany.
Armin KrausResearch Group "Magdeburger Arbeitsgemeinschaft für Forschung unter Raumfahrt- und Schwerelosigkeitsbedingungen" (MARS), Otto von Guericke University, 39106 Magdeburg, Germany.ORCID 0000-0001-6557-2163
Marcus KrügerDepartment of Microgravity and Translational Regenerative Medicine, Otto von Guericke University, 39106 Magdeburg, Germany.ORCID 0000-0003-1120-0246

Funding

German Space Agency at DLR 50WB2219
6 · The paper itself

Abstract

The formation of tumor spheroids on the random positioning machine (RPM) is a complex and important process, as it enables the study of metastasis ex vivo. However, this process is not yet understood in detail. In this study, we compared the RPM-induced spheroid formation of two cell types of lung carcinoma (NCI-H1703 squamous cell carcinoma cells and Calu-3 adenocarcinoma cells). While NCI-H1703 cells were mainly present as spheroids after 3 days of random positioning, Calu-3 cells remained predominantly as a cell layer. We found that two-dimensional-growing Calu-3 cells have less mucin-1, further downregulate their expression on the RPM and therefore exhibit a higher adhesiveness. In addition, we observed that Calu-3 cells can form spheroids, but they are unstable due to an imbalanced ratio of adhesion proteins (β

Indexed as

Estrogen Receptor alphaLung NeoplasmsSpheroids, CellularCadherinsCell AdhesionCell Line, TumorEstradiolEstrogensGene Expression Regulation, NeoplasticHumansIntegrin beta1Mucin-1CadherinsESR1 protein, humanEstradiolEstrogen Receptor alphaEstrogensIntegrin beta1MUC1 protein, humanMucin-1cell signalinglung cancerphenol redsimulated microgravitytumor spheroids

Identifiers

PMID39456226
PMCPMC11506229

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.