Evidence map›Paper›PMID 39455925›Full record

ArticleBMC genomics2024

Identification of the optimal reference genes for atrial fibrillation model established by iPSC-derived atrial myocytes.

Lei Li, Zijuan Zhao, Zihao Liu, Yuquan Tang, Tan Yang, Nailin Gong, Bing Liao, Yang Long, Yongmei Nie, Fengxu Yu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Lei Li *Department of Cardiovascular Surgery, The Affiliated Hospital of Southwest Medical University, NO25, Taiping Street, Luzhou City, 646000, China.
Zijuan Zhao *Department of Nursing, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, China.
Zihao LiuDepartment of Cardiovascular Surgery, The Affiliated Hospital of Southwest Medical University, NO25, Taiping Street, Luzhou City, 646000, China.
Yuquan TangDepartment of Cardiovascular Surgery, The Affiliated Hospital of Southwest Medical University, NO25, Taiping Street, Luzhou City, 646000, China.
Tan YangDepartment of Cardiovascular Surgery, The Affiliated Hospital of Southwest Medical University, NO25, Taiping Street, Luzhou City, 646000, China.
Nailin GongDepartment of Cardiovascular Surgery, The Affiliated Hospital of Southwest Medical University, NO25, Taiping Street, Luzhou City, 646000, China.
Bing LiaoDepartment of Cardiovascular Surgery, The Affiliated Hospital of Southwest Medical University, NO25, Taiping Street, Luzhou City, 646000, China.
Yang LongMetabolic Vascular Disease Key Laboratory of Sichuan Province, Luzhou, 646000, China.
Yongmei NieDepartment of Cardiovascular Surgery, The Affiliated Hospital of Southwest Medical University, NO25, Taiping Street, Luzhou City, 646000, China. nieyongmei@126.com.
Fengxu YuDepartment of Cardiovascular Surgery, The Affiliated Hospital of Southwest Medical University, NO25, Taiping Street, Luzhou City, 646000, China. yuluchuan@163.com.

Funding

Key Laboratory of Medical Electrophysiology of Ministry of Education KeyME- 2023-01Luzhou Science and Technology Bureau 2021LZXNYD-J26Luzhou Science and Technology Bureau 2021LZXNYD-Z07Sichuan Province Science and Technology Support Program 2022YFS0610Sichuan Province Science and Technology Support Program 2022YFS0617
6 · The paper itself

Abstract

backgroundAtrial fibrillation (AF) stands as a prevalent and detrimental arrhythmic disorder, characterized by intricate pathophysiological mechanisms. The availability of reliable and reproducible AF models is pivotal in unraveling the underlying mechanisms of this complex condition. Unfortunately, the researchers are still confronted with the absence of consistent in vitro AF models, hindering progress in this crucial area of research.

methodsHuman induced pluripotent stem cells derived atrial myocytes (hiPSC-AMs) were generated based on the GiWi methods and were verified by whole-cell patch clamp, immunofluorescent staining, and flow cytometry. Then hiPSC-AMs were employed to establish the AF model by HS. Whole-cell patch clamp technique and calcium imaging were used to identify the AF model. The stability of 29 reference genes was evaluated using delta-Ct, GeNorm, NormFinder, and BestKeeper algorithms;

resultsHiPSC-AMs displayed atrial myocyte action potentials and expressed the atrial-specific protein MLC-2 A and NR2F2, about 70% of the cardiomyocytes were MLC-2 A positive. After HS, hiPSC-AMs showed a significant increase in beating frequency, a shortened action potential duration, and increased calcium transient frequency. Of the 29 candidate genes, the top five most stably ranked genes were ABL1, RPL37A, POP4, RPL30, and EIF2B1. After normalization using ABL1, KCNJ2 was significantly upregulated in the AF model; Conclusions: In the hiPSC-AMs AF model established by HS, ABL1 provides greater normalization efficiency than commonly used GAPDH.

Indexed as

Atrial FibrillationHeart AtriaInduced Pluripotent Stem CellsMyocytes, CardiacAction PotentialsCell DifferentiationGene Expression ProfilingHumansReference StandardsAtrial fibrillationAtrial fibrillation modelElectrical stimulationInduced pluripotent stem cellsQuantitative real-time polymerase chain reactionReference gene

Identifiers

PMID39455925
PMCPMC11515253

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.