Evidence map›Paper›PMID 39455633›Full record

ArticleScientific reports2024

Mixed and membrane-separated culturing of synthetic cyanobacteria-yeast consortia reveals metabolic cross-talk mimicking natural cyanolichens.

Pavlo Bohutskyi, Kyle R Pomraning, Jackson P Jenkins, Young-Mo Kim, Brenton C Poirier, Michael J Betenbaugh, Jon K Magnuson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Pavlo BohutskyiEarth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, 99354, USA. pavlo.bohutskyi@pnnl.gov.
Kyle R PomraningEnergy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, 99354, USA.
Jackson P JenkinsDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
Young-Mo KimEarth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, 99354, USA.
Brenton C PoirierEarth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, 99354, USA.
Michael J BetenbaughDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
Jon K MagnusonEnergy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, 99354, USA.

Funding

Laboratory Directed Research and Development Program at Pacific Northwest National Laboratory Predictive Phenomics InitiativeU.S. Department of Energy Genomic Science Program DE-SC0019388
6 · The paper itself

Abstract

Metabolite exchange mediates crucial interactions in microbial communities, significantly impacting global carbon and nitrogen cycling. Understanding these chemically-mediated interactions is essential for elucidating natural community functions and developing engineered synthetic communities. This study investigated membrane-separated bioreactors (mBRs) as a novel tool to identify transient metabolites and their producers/consumers in mixed microbial communities. We compared three co-culture methods (direct mixed, 2-chamber mBR, and 3-chamber mBR) to grow a synthetic binary community of the cyanobacterium Synechococcus elongatus PCC 7942 and the fungus Rhodotorula toruloides NBRC 0880, as well as axenic S. elongatus. Despite not being natural lichen constituents, these organisms exhibited interactions resembling those in cyanolichens. S. elongatus fixed CO

Indexed as

BioreactorsCoculture TechniquesSynechococcusCyanobacteriaLichensMicrobial ConsortiaMicrobial InteractionsRhodotorulaCross-feedingMembrane-separated bioreactorMetabolite exchangeMicrobial communityPhototroph-heterotroph co-cultureSynthetic lichen

Identifiers

PMID39455633
PMCPMC11511929

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.