Evidence map›Paper›PMID 39454956›Full record

ArticleThe Journal of biological chemistry2024

Identification of a staphylococcal dipeptidase involved in the production of human body odor.

Reyme Herman, Bethan Kinniment-Williams, Michelle Rudden, Alexander Gordon James, Anthony J Wilkinson, Barry Murphy, Gavin H Thomas

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Human Body Malodor and Deodorants: The Present and the Future.International journal of molecular sciences · 2025
    Review
  3. High-resolutionMicrobial genomics · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Reyme HermanDepartment of Biology, University of York, York, UK; York Biomedical Research Institute, University of York, York, UK.
Bethan Kinniment-WilliamsYork Biomedical Research Institute, University of York, York, UK; Hull York Medical School, University of York, York, UK.
Michelle RuddenDepartment of Biology, University of York, York, UK; School of Life Sciences, University of Hull, Hull, UK.
Alexander Gordon JamesUnilever, Colworth Science Park, Bedfordshire, UK.
Anthony J WilkinsonYork Structural Biology Laboratory, Department of Chemistry, University of York, York, UK.
Barry MurphyUnilever Research & Development, Port Sunlight Laboratory, Merseyside, UK.
Gavin H ThomasDepartment of Biology, University of York, York, UK; York Biomedical Research Institute, University of York, York, UK. Electronic address: gavin.thomas@york.ac.uk.

Funding

ChimeraX -- Next Generation Visualization and Analysis Software for Multiscale ModelingR01GM129325 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FERRIN, THOMAS E · 2018 to 2025
$5.2M
NIGMS NIH HHS R01 GM129325
6 · The paper itself

Abstract

The production of human body odor is the result of the action of commensal skin bacteria, including Staphylococcus hominis, acting to biotransform odorless apocrine gland secretions into volatile chemicals like thioalcohols such as 3-methyl-3-sulphanylhexan-1-ol (3M3SH). As the secreted odor precursor Cys-Gly-3M3SH contains a dipeptide, yet the final enzyme in the biotransformation pathway only functions on Cys-3M3SH, we sought to identify the remaining step in this human-adapted biochemical pathway using a novel coupled enzyme assay. Purification of this activity from S. hominis extracts led to the identification of the M20A-family PepV peptidase (ShPepV) as the primary Cys-Gly-3M3SH dipeptidase. To establish whether this was a primary substrate for PepV, the recombinant protein was purified and demonstrated broad activity against diverse dipeptides. The binding site for Cys-Gly-3M3SH was predicted using modeling, which suggested mutations that might accommodate this ligand more favorably. Indeed, a D437A resulted in an almost sixfold increase in the k

Indexed as

DipeptidasesOdorantsBacterial ProteinsDipeptidesHexanolsHumansRecombinant ProteinsStaphylococcus hominisSubstrate SpecificityBacterial ProteinsDipeptidasesDipeptidesHexanolsRecombinant ProteinsCys-Gly-3M3SHenzyme kineticsenzyme structuremalodormetalloenzymepeptidasepeptidesStaphylococcus hominis

Identifiers

PMID39454956
PMCPMC11742315

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.