What Quality Suffices for Nanopore Metabarcoding? Reconsidering Methodology and Ectomycorrhizae in Decaying

Glen Dierickx, Lowie Tondeleir, Pieter Asselman, Kris Vandekerkhove, Annemieke Verbeken

Abstract read

In one paragraph

Article in Journal of fungi (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Tree identity shapes composition, substrate shifts dominance in deadwood fungi of an old-growth lowland beech forest.IMA fungus · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Glen DierickxResearch Group Mycology, Ghent University, 9000 Gent, Belgium.ORCID 0000-0001-5387-8544

Lowie TondeleirResearch Group Mycology, Ghent University, 9000 Gent, Belgium.ORCID 0009-0008-5951-1709

Pieter AsselmanResearch Group Mycology, Ghent University, 9000 Gent, Belgium.

Kris VandekerkhoveResearch Institute for Nature and Forest, 9500 Geraardsbergen, Belgium.ORCID 0000-0003-1954-692X

Annemieke VerbekenResearch Group Mycology, Ghent University, 9000 Gent, Belgium.

Funding

Research Foundation - Flanders 1126223N

6 · The paper itself

Abstract

Nanopore raw read accuracy has improved to over 99%, making it a potential tool for metabarcoding. For broad adoption, guidelines on quality filtering are needed to ensure reliable taxonomic unit recovery. This study aims to provide those guidelines for a fungal metabarcoding context and to apply them to a case study of ectomycorrhizae in the decaying bark of

Indexed as

deadwoodectomycorrhizaeNano pipelineFagus sylvaticametabarcodingNanoporenatural regenerationPhred scorequality filtering

Identifiers

PMID39452660

DOI10.3390/jof10100708

PMCPMC11508852

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.