Evidence map›Paper›PMID 39452250›Full record

ArticleAntibiotics (Basel, Switzerland)2024

Validation of Recombinase Polymerase Amplification with In-House Lateral Flow Assay for

Naeem Ullah, Nutchaba Suchanta, Umaporn Pimpitak, Pitak Santanirand, Nutthee Am-In, Nuntaree Chaichanawongsaroj

Abstract read
In one paragraph

Article in Antibiotics (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Naeem UllahCenter of Excellence for Innovative Diagnosis of Antimicrobial Resistance, Department of Transfusion Medicine and Clinical Microbiology, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Nutchaba SuchantaCenter of Excellence for Innovative Diagnosis of Antimicrobial Resistance, Department of Transfusion Medicine and Clinical Microbiology, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Umaporn PimpitakInstitute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok 10330, Thailand.
Pitak SantanirandDepartment of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.ORCID 0000-0002-3914-1367
Nutthee Am-InDepartment of Obstetrics Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.ORCID 0000-0002-3873-6369
Nuntaree ChaichanawongsarojCenter of Excellence for Innovative Diagnosis of Antimicrobial Resistance, Department of Transfusion Medicine and Clinical Microbiology, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.ORCID 0000-0002-0717-5466

Funding

Thailand Science research and Innovation Fund Chulalongkorn University FOOD66370008
6 · The paper itself

Abstract

BACKGROUND/

objectivesThe emergence of the mobilized colistin resistance 1 (

methodsThe colistin agar test and broth microdilution were employed to screen 152

resultsThe RPA-in-house LFA was completed within 35 min (20 min of amplification and 5-15 min on LFA detection) at 37 °C. The sensitivity, specificity, and accuracy were entirely 100% in concordance with PCR results. No cross-reactivity was detected with seven common pathogenic bacteria or other

conclusionsTherefore, the in-house RPA-LFA serves as a point-of-care testing tool that is rapid, simple, and portable, facilitating effective surveillance of colistin resistance in both veterinary and clinical settings, thereby enhancing health outcomes.

Indexed as

Escherichia colilateral flow assaymobile colistin resistance 1 (mcr-1) genepoint-of-care testingrecombinase polymerase amplification

Identifiers

PMID39452250
PMCPMC11505259

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.