ArticleInfectious disease reports2024
Application of PCR-Based Techniques for the Identification of Genetic Fingerprint Diversity of Dominant Bacteria in Fecal Samples of Children with Diarrhea in Vietnam.
Article in Infectious disease reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Development of Loop-Mediated Isothermal Amplification Assay for the Detection of aaic Positive Enteroaggregative Escherichia coli (EAEC).Applied biochemistry and biotechnology · 2026Article
- Understanding the bacteriome, phageome and phage-associated bacteriome in healthy Vietnamese children under two years of age.Archives of microbiology · 2026Article
- PCR-Based Versus Conventional Stool Testing in Hospitalized Patients with Diarrhea: Diagnostic Yield, Clinical Impact, and Stewardship Implications.Microorganisms · 2025Article
- Diversity and Functional Predictions of Gut Microbiota in Vietnamese Children Aged 6-24 Months with Persistent Diarrhea of Unknown Etiology.Pathogens (Basel, Switzerland) · 2025Article
- Metagenomic Analysis of the Gastrointestinal Phageome and Incorporated Dysbiosis in Children with Persistent Diarrhea of Unknown Etiology in Vietnam.Pathogens (Basel, Switzerland) · 2025Article
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Authors and funding
10 authors.
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Abstract
In Vietnam, diarrhea, especially persistent diarrhea, is one of the most common diseases in children, while a significant proportion of cases are negative with pathogens; thus, there is an urgent need to understand gut bacterial dysbiosis. In this study, bacteria in the fecal samples of five healthy and ten diarrheal children were separated from other residues, then adopted to extract their metagenomic DNA for evaluating their diversity based on V3 and V6-V8 regions and the 16S rRNA gene by PCR-RFLP and PCR-DGGE. As a result, bacterial metagenomic DNAs with high quality, quantity and diversity were successfully extracted using a GeneJET kit and a chemical protocol. A sequence analysis of 73 representative DNA fragments from gels indicated a remarkable bacterial dysbiosis in all groups of diarrhea. Viral diarrhea was characterized by extremely reduced bacterial diversity with the blossom of
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