Non-Invasive On-Off Fluorescent Biosensor for Endothelial Cell Detection.

Qingyun Jiang, Shuai Shao, Na Li, Zhengyao Zhang, Bo Liu

Abstract read

In one paragraph

Article in Biosensors, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Qingyun JiangCancer Hospital of Dalian University of Technology, Shenyang 110042, China.ORCID 0000-0003-2401-4880

Shuai ShaoCancer Hospital of Dalian University of Technology, Shenyang 110042, China.

Na LiCancer Hospital of Dalian University of Technology, Shenyang 110042, China.

Zhengyao ZhangSchool of Chemical Engineering, Ocean and Life Sciences, Dalian University of Technology, Panjin 124221, China.

Bo LiuCancer Hospital of Dalian University of Technology, Shenyang 110042, China.ORCID 0000-0002-5375-0505

Funding

National Natural Science Foundation of China 32071252

6 · The paper itself

Abstract

For rapid and convenient detection of living endothelial cells (ECs) specifically without immunostaining, we developed a biosensor based on turn-on fluorescent protein, named LV-EcpG. It includes a high-affinity peptide E12P obtained through phage display technology for specifically recognizing ECs and a turn-on EGFP fused with two linker peptides. The "on-off" switching mechanism of this genetically encoded fluorescent protein-based biosensor (FPB) ensured that fluorescence signals were activated only when binding with ECs, thus enabling these FPB characters for direct, visual, and non-invasive detection of ECs. Its specificity and multicolor imaging capability established LV-EcpG as a powerful tool for live EC research, with significant potential for diagnosing and treating cardiovascular diseases and tumor angiogenesis.

Indexed as

Biosensing TechniquesEndothelial CellsGreen Fluorescent ProteinsHumansHuman Umbilical Vein Endothelial Cellsenhanced green fluorescent proteinGreen Fluorescent Proteinsaffinity peptideendothelial cells (ECs)genetically encoded fluorescent protein-based biosensors (FPBs)non-invasivephage display

Identifiers

PMID39451702

DOI10.3390/bios14100489

PMCPMC11506521

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.