Evidence map›Paper›PMID 39447577›Full record

ArticleCell chemical biology2024

RNA infrastructure profiling illuminates transcriptome structure in crowded spaces.

Lu Xiao, Linglan Fang, Wenrui Zhong, Eric T Kool

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell chemical biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Alzheimer's Disease: A Suitable Case for Treatment with Precision Medicine?Medical principles and practice : international journal of the Kuwait University, Health Science Centre · 2024
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors.

Lu XiaoDepartment of Chemistry, Stanford University, Stanford, CA 94305, USA.
Linglan FangDepartment of Chemistry, Stanford University, Stanford, CA 94305, USA.
Wenrui ZhongDepartment of Chemistry, Stanford University, Stanford, CA 94305, USA.
Eric T KoolDepartment of Chemistry, Stanford University, Stanford, CA 94305, USA; Sarafan ChEM-H Institute, Stanford University, Stanford, CA 94305, USA. Electronic address: kool@stanford.edu.

Funding

Transcriptome Analysis with RNA-Reactive ProbesR35GM145357 · NIGMS · STANFORD UNIVERSITY · PI ERIC T. KOOL · 2022 to 2026
$3.0M
NIGMS NIH HHS R35 GM145357
6 · The paper itself

Abstract

RNAs fold into compact structures and undergo protein interactions in cells. These occluded environments can block reagents that probe the underlying RNAs. Probes that can analyze structure in crowded settings can shed light on RNA biology. Here, we employ 2'-OH-reactive probes that are small enough to access folded RNA structure underlying close molecular contacts within cells, providing considerably broader coverage for intracellular RNA structural analysis. The data are analyzed first with well-characterized human ribosomal RNAs and then applied transcriptome-wide to polyadenylated transcripts. The smallest probe acetylimidazole (AcIm) yields 80% greater structural coverage than larger conventional reagent NAIN3, providing enhanced structural information in hundreds of transcripts. The acetyl probe also provides superior signals for identifying m

Indexed as

TranscriptomeHumansImidazolesNucleic Acid ConformationRNARNA, RibosomalImidazolesRNARNA, Ribosomalacylationcrowded spaceinfrastructureN(6)-methyladenosineRNA

Identifiers

PMID39447577
PMCPMC12814655

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.