Evidence map›Paper›PMID 39444155›Full record

ArticleNephrology (Carlton, Vic.)2024

Identification of circulating microbial DNA and its association with kidney function in patients with diabetic kidney disease.

Hoang Thuy Linh, Megumi Oshima, Keisuke Sako, Masahiro Konishi, Daiki Hayashi, Hajime Sanada, Takahiro Yuasa, Akihiko Koshino, Keisuke Horikoshi, Taichiro Minami and 13 more

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In one paragraph

Article in Nephrology (Carlton, Vic.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors.

Hoang Thuy LinhDepartment of Nephrology and Rheumatology, Kanazawa University, Japan.ORCID https://orcid.org/0000-0001-7860-5219
Megumi OshimaDepartment of Nephrology and Rheumatology, Kanazawa University, Japan.
Keisuke SakoDepartment of Nephrology and Rheumatology, Kanazawa University, Japan.
Masahiro KonishiDepartment of Nephrology and Rheumatology, Kanazawa University, Japan.
Daiki HayashiDepartment of Nephrology and Rheumatology, Kanazawa University, Japan.
Hajime SanadaDepartment of Nephrology and Rheumatology, Kanazawa University, Japan.
Takahiro YuasaDepartment of Nephrology and Rheumatology, Kanazawa University, Japan.
Akihiko KoshinoDepartment of Nephrology and Rheumatology, Kanazawa University, Japan.
Keisuke HorikoshiDepartment of Nephrology and Rheumatology, Kanazawa University, Japan.
Taichiro MinamiDepartment of Nephrology and Rheumatology, Kanazawa University, Japan.
Shunsuke TsugeDepartment of Nephrology and Rheumatology, Kanazawa University, Japan.
Akira TamaiDepartment of Nephrology and Rheumatology, Kanazawa University, Japan.
Shiori NakagawaDepartment of Nephrology and Rheumatology, Kanazawa University, Japan.
Ryo NishiokaDepartment of Nephrology and Rheumatology, Kanazawa University, Japan.
Takeshi ZoshimaDepartment of Nephrology and Rheumatology, Kanazawa University, Japan.
Kiyoaki ItoDepartment of Nephrology and Rheumatology, Kanazawa University, Japan.
Ichiro MizushimaDepartment of Nephrology and Rheumatology, Kanazawa University, Japan.
Tadashi ToyamaDepartment of Nephrology, University of Fukui School of Medical Sciences, Fukui, Japan.
Norihiko SakaiDepartment of Nephrology and Rheumatology, Kanazawa University, Japan.
Shinji KitajimaDepartment of Nephrology and Rheumatology, Kanazawa University, Japan.ORCID https://orcid.org/0000-0002-6532-6496
Miho ShimizuDepartment of Nephrology and Rheumatology, Kanazawa University, Japan.
Takashi WadaDepartment of Nephrology and Rheumatology, Kanazawa University, Japan.
Yasunori IwataDepartment of Nephrology and Rheumatology, Kanazawa University, Japan.

Funding

Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research (KAKENHI) 23K15246
6 · The paper itself

Abstract

aimRecently, substantial studies have been accumulated to indicate the important role of gut microbiota in diabetic kidney disease (DKD). The abnormal change of bacterial-derived products could imply specific injuries or play beneficial or harmful roles in DKD progression. In this study, we examined the presence and contribution of the Klebsiella oxytoca gene in the circulation of patients with DKD.

methodWe enrolled a total of 16 healthy participants, 17 patients with DKD, 5 patients with DKD requiring haemodialysis (HD), and 7 patients with CKD without diabetes. Bacterial-derived DNA (16S rDNA and a specific K. oxytoca gene) in the blood was detected using droplet digital PCR, then investigated the relationship with clinical characteristics.

resultsWe identified an increase in K. oxytoca genes in the blood of DKD patients. Interestingly, blood K. oxytoca copies and K. oxytoca/ 16S DNA ratio correlated with higher blood creatinine and BUN levels together with lower eGFR in DKD patients. K. oxytoca levels were also associated with higher neutrophil percentage, lower lymphocyte frequency, and increased neutrophil-to-lymphocyte ratio.

conclusionCollectively, the presence of the K. oxytoca gene in the circulation could serve as a biomarker reflecting reduced renal function in DKD patients.

Indexed as

Diabetic NephropathiesDNA, BacterialGlomerular Filtration RateKlebsiella oxytocaAdultAgedBiomarkersCase-Control StudiesFemaleHumansKidneyMaleMiddle AgedRenal DialysisRNA, Ribosomal, 16SBiomarkersDNA, BacterialRNA, Ribosomal, 16Scirculating microbial DNAdiabetic kidney diseaseKlebsiella oxytoca

Identifiers

PMID39444155
PMCPMC13546966

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.