Evidence map›Paper›PMID 39444023›Full record

ArticleBiology direct2024

Deciphering the impact of MreB on the morphology and pathogenicity of the aquatic pathogen Spiroplasma eriocheiris.

Rong Li, Xiaohui Cao, Jiaxin Chen, Tingting He, Yan Zhang, Wen Wang, Yaqi Wang, Yifei Wang, Yanyan Qiu, Mengji Xie and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology direct, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Rong Li *Institute of Pathogenic Biology, Basic Medical School,University of South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang, Hunan, 421001, China.
Xiaohui Cao *Jiangsu Marine Fisheries Research Institute, Nantong, Jiangsu, 226007, China.
Jiaxin Chen *Institute of Pathogenic Biology, Basic Medical School,University of South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang, Hunan, 421001, China.
Tingting He *Shaoxing Center for Disease Control and Prevention, 276 Century Street, Shaoxing, Zhejiang Province, 312000, China.
Yan ZhangInstitute of Pathogenic Biology, Basic Medical School,University of South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang, Hunan, 421001, China.
Wen WangJiangsu Key Laboratory for Biodiversity and Biotechnology, Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, Jiangsu, China.
Yaqi WangInstitute of Pathogenic Biology, Basic Medical School,University of South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang, Hunan, 421001, China.
Yifei WangInstitute of Pathogenic Biology, Basic Medical School,University of South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang, Hunan, 421001, China.
Yanyan QiuInstitute of Pathogenic Biology, Basic Medical School,University of South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang, Hunan, 421001, China.
Mengji XieInstitute of Pathogenic Biology, Basic Medical School,University of South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang, Hunan, 421001, China.
Kailin ShiInstitute of Pathogenic Biology, Basic Medical School,University of South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang, Hunan, 421001, China.
Yuhua XuInstitute of Pathogenic Biology, Basic Medical School,University of South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang, Hunan, 421001, China.
Siyuan ZhangInstitute of Pathogenic Biology, Basic Medical School,University of South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang, Hunan, 421001, China.
Peng LiuInstitute of Pathogenic Biology, Basic Medical School,University of South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang, Hunan, 421001, China. pengliu@live.cn.

Funding

Hunan Provincial College Students' innovation and Entrepreneurship Training Program S202310555204Hunan Provincial College Students' innovation and Entrepreneurship Training Program S202312650005Hunan Provincial College Students' innovation and Entrepreneurship Training Program S202410555051Hunan Provincial College Students' innovation and Entrepreneurship Training Program X202410555207Hunan Provincial College Students' innovation and Entrepreneurship Training Program X202410555214National Natural Science Foundation of China 32370209Natural Science Foundation of Hunan Province, China 2023JJ30503Scientific Research Foundation of Hunan Provincial Education Department 22A0297
6 · The paper itself

Abstract

backgroundSpiroplasma eriocheiris has been proved to be a pathogen causing tremor disease of Eriocheir sinensis, it is also infectious to other aquatic crustaceans, resulting in a serious threat on the sustainable development of the aquaculture industry. S. eriocheiris is a helical-shaped microbe without a cell wall, and its motility is related to the cytoskeleton protein MreB which belongs to the actin superfamily and has five MreB homologs.

resultsIn this study, we purified MreB3, MreB4 and MreB5, and successfully prepared monoclonal antibodies. After S. eriocheiris treated with actin stabilizator Phalloidin and inhibitors A22, we found that Phalloidin and A22 affect the S. eriocheiris morphology by altering MreB expression. We confirmed that the ability of S. eriocheiris to invade E. sinensis was increased after treatment with Phalloidin, including that the morphology of E. sinensis blood lymphocytes was deteriorated, blood lymphocytes viability was decreased, peroxidase activity and cell necrosis were increased. On the contrary, the pathogenicity of S. eriocheiris decreased after treatment with A22.

conclusionsOur findings suggest that the MreB protein in S. eriocheiris plays a crucial role in its morphology and pathogenicity, providing new insights into potential strategies for the prevention and control of S. eriocheiris infections.

Indexed as

SpiroplasmaAnimalsBacterial ProteinsBrachyuraVirulenceBacterial ProteinsA22MreBPathogenicity and morphologyPhalloidinSpiroplasma eriocheiris

Identifiers

PMID39444023
PMCPMC11515736

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.