Evidence map›Paper›PMID 39443409›Full record

ArticleGeneral thoracic and cardiovascular surgery2025

Intraoperative rapid immunohistochemistry of microsatellite instability using non-contact alternating current electric field mixing.

Kazuhiro Imai, Naoki Yanagawa, Hajime Saito, Hiroshi Nanjo, Yuki Wakamatsu, Shinogu Takashima, Tsubasa Matsuo, Shoji Kuriyama, Tamotsu Sugai, Yoshihiro Minamiya

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Article in General thoracic and cardiovascular surgery, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Kazuhiro ImaiDepartment of Thoracic Surgery, Akita University Graduate School of Medicine, 1-1-1 Hondo, Akita, 010-8543, Japan. i-karo@mui.biglobe.ne.jp.ORCID http://orcid.org/0000-0003-4490-7862
Naoki YanagawaDepartment of Molecular Diagnostic Pathology, Iwate Medical University, Yahaba-cho, Iwate, Japan.
Hajime SaitoDepartment of Thoracic Surgery, Iwate Medical University, Yahaba-cho, Iwate, Japan.
Hiroshi NanjoDepartment of Pathology, Akita University Graduate School of Medicine, Akita, Japan.
Yuki WakamatsuDepartment of Thoracic Surgery, Akita University Graduate School of Medicine, 1-1-1 Hondo, Akita, 010-8543, Japan.
Shinogu TakashimaDepartment of Thoracic Surgery, Akita University Graduate School of Medicine, 1-1-1 Hondo, Akita, 010-8543, Japan.
Tsubasa MatsuoDepartment of Thoracic Surgery, Akita University Graduate School of Medicine, 1-1-1 Hondo, Akita, 010-8543, Japan.
Shoji KuriyamaDepartment of Thoracic Surgery, Akita University Graduate School of Medicine, 1-1-1 Hondo, Akita, 010-8543, Japan.
Tamotsu SugaiDepartment of Molecular Diagnostic Pathology, Iwate Medical University, Yahaba-cho, Iwate, Japan.
Yoshihiro MinamiyaDepartment of Thoracic Surgery, Akita University Graduate School of Medicine, 1-1-1 Hondo, Akita, 010-8543, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesTumors caused by failure of the DNA-mismatch repair system generally show microsatellite instability (MSI). High-frequency MSI cancers have been shown to be susceptible to immuno-oncology therapies. The aim of this study was to evaluate the clinical reliability of a rapid immunohistochemistry (IHC) technique for intraoperatively assessing molecular status through detection of tumoral deficiencies in the expression of mismatch repair proteins (dMMR; MLH1, MSH2, MSH6, and PMS2).

methodsThe rapid IHC method uses non-contact alternating current (AC) mixing to achieve more rapid/stable staining within a minimum of 13 min during surgery. Sixteen formalin-fixed paraffin-embedded (FFPE) tumor samples from 3 dMMR patients with Lynch syndrome and 6 FFPE samples from 6 dMMR-cancer patients were collected to establish an IHC protocol for MMR proteins. Next, 26 surgical patients treated and whose MSI status was determined using PCR-based tests were retrospectively analyzed. The concordance of dMMR diagnoses for thoracic tumors between the conventional (frozen section (FS)- and FFPE-IHCs) and rapid AC-mixing IHC with FSs were compared.

resultsA rapid IHC protocol using primary antibodies against four MMR proteins (mixed 5-10 min) was established (entire process within 40 min). The concordance rate for MMR-IHC between the conventional and rapid IHC was 100%. dMMR diagnoses including an MSI-high pulmonary sarcoma patient entirely matched between FS- and FFPE-IHC.

conclusionRapid MMR-IHC could potentially serve as a clinical tool for intraoperative determination of tumor MSI/dMMR status. AC-mixing technology will contribute to improving pathological diagnostic capability through the development of an original and innovative rapid IHC.

Indexed as

Biomarkers, TumorColorectal Neoplasms, Hereditary NonpolyposisDNA Mismatch RepairImmunohistochemistryMicrosatellite InstabilityAdultAgedDNA-Binding ProteinsFemaleFrozen SectionsHumansMaleMiddle AgedMismatch Repair Endonuclease PMS2MutL Protein Homolog 1MutS Homolog 2 ProteinBiomarkers, TumorDNA-Binding ProteinsG-T mismatch-binding proteinMismatch Repair Endonuclease PMS2MLH1 protein, humanMSH2 protein, humanMutL Protein Homolog 1MutS Homolog 2 ProteinPMS2 protein, humanAlternating current electric field mixingCancerDeficient DNA-mismatch repairMicrosatellite instabilityRapid immunocytochemistry

Identifiers

PMID39443409

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.