Evidence map›Paper›PMID 39441441›Full record

ArticleBulletin of experimental biology and medicine2024

Identification of Giant Isoforms of Obscurin in Rat Striated Muscles Using Polyclonal Antibodies.

Y V Gritsyna, V K Zhalimov, T A Uryupina, A D Ulanova, A G Bobylev, I M Vikhlyantsev

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Article in Bulletin of experimental biology and medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Y V GritsynaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia.
V K ZhalimovInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia.
T A UryupinaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia.
A D UlanovaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia.
A G BobylevInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia.
I M VikhlyantsevInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia. ivanvikhlyantsev@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Using produced polyclonal antibodies specific to the N-terminal sequence (residues 61-298) of rat obscurin, we investigated the isoform composition of this protein in 4 striated muscles: myocardium of the left ventricle, diaphragm, skeletal m. gastrocnemius (containing mainly fast fibers), and m. soleus (containing mainly slow fibers). The m. gastrocnemius, m. soleus, and diaphragm were found to have 2 giant isoforms of obscurin: a smaller A-isoform and a larger B-isoform. Their molecular weights were ~870 and ~1150 kDa in the diaphragm and m. gastrocnemius and ~880 and ~1130 kDa in m. soleus, respectively. The B-isoform to A-isoform ratio was 1:3 in the diaphragm and m. soleus and 1:4 in the m. gastrocnemius. In the left-ventricular myocardium, A-isoform of obscurin with a molecular weight of ~880 kDa was found. No other obscurin isoforms or their fragments within the molecular weight range of 10 up to ~800 kDa were revealed in the investigated rat striated muscles. The antibodies produced are recommended for research into qualitative and quantitative changes of giant obscurin isoforms in rat striated muscles in the norm and during the development of pathological processes.

Indexed as

AntibodiesMuscle, StriatedMyocardiumProtein IsoformsAnimalsDiaphragmGuanine Nucleotide Exchange FactorsHeart VentriclesMaleMuscle ProteinsMuscle, SkeletalProtein Serine-Threonine KinasesRatsRats, WistarRho Guanine Nucleotide Exchange FactorsAntibodiesGuanine Nucleotide Exchange FactorsMuscle ProteinsObscn protein, ratProtein IsoformsProtein Serine-Threonine KinasesRho Guanine Nucleotide Exchange Factorsstriated muscles; giant muscle proteins; obscurin; obscurin gene (OBSCN)

Identifiers

PMID39441441

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.