ArticleExploration (Beijing, China)2024
Detection of biological loads in sewage using the automated robot-driven photoelectrochemical biosensing platform.
Article in Exploration (Beijing, China), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The trial behind it
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Who cites it
8 citing papers in PubMed.
- Detection of Respiratory Viruses in Wastewater Samples Using Commercial Multiplex PCR Assays.Viruses · 2026Article
- Indiscriminate Trans-Cleavage Activity of CRISPR/SuCas12a2 Enables Sensitive Detection of SARS-CoV-2.ACS sensors · 2026Article
- Microfluidic-optical integrated portable platform with handheld pump for wash-free plasmonic detection of thrombin and SARS-CoV-2 spike protein.Microsystems & nanoengineering · 2026Article
- The saturation effect of body mass index on femoral neck bone mineral density in a sample of apparently healthy older Syrians: A cross-sectional study.The Journal of international medical research · 2026Article
- Real-Time Feedback Strategically Regulates Optoelectronics for Customized Optogenetic Spinal Cord Regeneration.Exploration (Beijing, China) · 2026Article
- Catalytic Hairpin Assembly-Propelled Weak-Inputs-Strong-Outputs (CP-WISO) DNA Logic Nanodevices with Orthogonal Design and Contrary Logic Responses.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Real-Time Intracellular Monitoring of miRNA Dynamics during Induced Pluripotent Stem Cell Neuronal Differentiation via Plasmon-Enhanced Nanobiosensing.Nano letters · 2025Article
- Detection of biological loads in sewage using the automated robot-driven photoelectrochemical biosensing platform.Exploration (Beijing, China) · 2024Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Real-time polymerase chain reaction (RT-PCR) remains the most prevalent molecular detection technology for sewage analysis but is plagued with numerous disadvantages, such as time consumption, high manpower requirements, and susceptibility to false negatives. In this study, an automated robot-driven photoelectrochemical (PEC) biosensing platform is constructed, that utilizes the CRISPR/Cas12a system to achieve fast, ultrasensitive, high specificity detection of biological loads in sewage. The Shennong-1 robot integrates several functional modules, involving sewage sampling and pretreatment to streamline the sewage monitoring. A screen-printed electrode is employed with a vertical graphene-based working electrode and enhanced with surface-deposited Au nanoparticles (NPs). CdTe/ZnS quantum dots (QDs) are further fabricated through the double-stranded DNA (dsDNA) anchored on Au NPs. Using the cDNA template of Omicron BA.5 spike gene as a model, the PEC biosensor demonstrates excellent analytical performance, with a lower detection limit of 2.93 × 10
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Registered trials
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