Evidence map›Paper›PMID 39438484›Full record

ArticleNature communications2024

Structuring lipid nanoparticles, DNA, and protein corona into stealth bionanoarchitectures for in vivo gene delivery.

Serena Renzi, Luca Digiacomo, Daniela Pozzi, Erica Quagliarini, Elisabetta Vulpis, Maria Valeria Giuli, Angelica Mancusi, Bianca Natiello, Maria Gemma Pignataro, Gianluca Canettieri and 17 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

27 authors.

Serena Renzi *Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy.
Luca Digiacomo *Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy.ORCID 0000-0003-3362-7160
Daniela Pozzi *Department of Molecular Medicine, Sapienza University of Rome, Rome, Italy.
Erica QuagliariniDepartment of Molecular Medicine, Sapienza University of Rome, Rome, Italy.
Elisabetta VulpisDepartment of Molecular Medicine, Sapienza University of Rome, Rome, Italy.
Maria Valeria GiuliDepartment of Medico-Surgical Sciences and Biotechnology, Sapienza University of Rome, Laboratory affiliated to Istituto Pasteur Italia-Fondazione Cenci Bolognetti, Latina, Italy.ORCID 0000-0003-2132-9369
Angelica MancusiDepartment of Molecular Medicine, Sapienza University of Rome, Rome, Italy.
Bianca NatielloDepartment of Molecular Medicine, Sapienza University of Rome, Rome, Italy.
Maria Gemma PignataroDepartment of Radiological, Oncological and Pathological Sciences, Sapienza University of Rome, Rome, Italy.
Gianluca CanettieriDepartment of Molecular Medicine, Sapienza University of Rome, Rome, Italy.ORCID 0000-0001-6694-2613
Laura Di MagnoDepartment of Molecular Medicine, Sapienza University of Rome, Rome, Italy.
Luca PesceNEST, Scuola Normale Superiore, Pisa, Italy.ORCID 0000-0002-5863-399X
Valentina De LorenziNEST, Scuola Normale Superiore, Pisa, Italy.ORCID 0000-0002-7429-6524
Samuele GhignoliNEST, Scuola Normale Superiore, Pisa, Italy.
Luisa LoconteDepartment of Molecular Medicine, Sapienza University of Rome, Rome, Italy.
Carmela Maria MontoneDepartment of Chemistry, Sapienza University of Rome, Rome, Italy.
Anna Laura CapriottiDepartment of Chemistry, Sapienza University of Rome, Rome, Italy.
Aldo LaganàDepartment of Chemistry, Sapienza University of Rome, Rome, Italy.
Carmine NicolettiUnit of Histology and Medical Embryology, Department of Anatomy, Histology, Forensic Medicine and Orthopedics, Sapienza University of Rome, Rome, Italy.ORCID 0000-0001-5896-2040
Heinz AmenitschInstitute of Inorganic Chemistry, Graz University of Technology, Graz, Austria.
Marco RossiDepartment of Basic and Applied Sciences for Engineering and Center for Nanotechnology Applied to Engineering (CNIS), Sapienza University of Rome, Rome, Italy.ORCID 0000-0001-7603-1805
Francesco MuraDepartment of Basic and Applied Sciences for Engineering and Center for Nanotechnology Applied to Engineering (CNIS), Sapienza University of Rome, Rome, Italy.
Giacomo ParisiDepartment of Basic and Applied Sciences for Engineering and Center for Nanotechnology Applied to Engineering (CNIS), Sapienza University of Rome, Rome, Italy.
Francesco CardarelliNEST, Scuola Normale Superiore, Pisa, Italy.ORCID 0000-0003-3049-5940
Alessandra ZingoniDepartment of Molecular Medicine, Sapienza University of Rome, Rome, Italy. alessandra.zingoni@uniroma1.it.ORCID 0000-0003-0906-1566
Saula ChecquoloDepartment of Medico-Surgical Sciences and Biotechnology, Sapienza University of Rome, Laboratory affiliated to Istituto Pasteur Italia-Fondazione Cenci Bolognetti, Latina, Italy. saula.checquolo@uniroma1.it.ORCID 0000-0001-5109-8392
Giulio CaraccioloDepartment of Molecular Medicine, Sapienza University of Rome, Rome, Italy. giulio.caracciolo@uniroma1.it.ORCID 0000-0002-8636-4475

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lipid nanoparticles (LNPs) play a crucial role in addressing genetic disorders, and cancer, and combating pandemics such as COVID-19 and its variants. Yet, the ability of LNPs to effectively encapsulate large-size DNA molecules remains elusive. This is a significant limitation, as the successful delivery of large-size DNA holds immense potential for gene therapy. To address this gap, the present study focuses on the design of PEGylated LNPs, incorporating large-sized DNA, departing from traditional RNA and ionizable lipids. The resultant LNPs demonstrate a unique particle morphology. These particles were further engineered with a DNA coating and plasma proteins. This multicomponent bionanoconstruct exhibits enhanced transfection efficiency and safety in controlled laboratory settings and improved immune system evasion in in vivo tests. These findings provide valuable insights for the design and development of bionanoarchitectures for large-size DNA delivery, opening new avenues for transformative gene therapies.

Indexed as

COVID-19DNAGenetic TherapyGene Transfer TechniquesNanoparticlesProtein CoronaAnimalsFemaleHumansLipidsLiposomesMiceParticle SizePolyethylene GlycolsSARS-CoV-2TransfectionDNALipid NanoparticlesLipidsLiposomesPolyethylene GlycolsProtein Corona

Identifiers

PMID39438484
PMCPMC11496629

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.