Evidence map›Paper›PMID 39436498›Full record

ArticleHead and neck pathology2024

Uncommon and Challenging Phenotypes of High-Risk Human Papillomavirus-Associated Head and Neck Carcinomas Revealed by High-Throughput Studies.

Alex P Tannenbaum, Taja Lozar, Changxue Lu, Megan Schumacher, Athena Golfinos, Huy Q Dinh, Natalie Taylor, Randall J Kimple, David Yang, Paul M Harari and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Head and neck pathology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Alex P TannenbaumDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, 53705, USA.
Taja LozarMcArdle Laboratory for Cancer Research, Madison, WI, 53705, USA.
Changxue LuBrady Urological Institute, The Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA.
Megan SchumacherBrady Urological Institute, The Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA.
Athena GolfinosMcArdle Laboratory for Cancer Research, Madison, WI, 53705, USA.
Huy Q DinhMcArdle Laboratory for Cancer Research, Madison, WI, 53705, USA.
Natalie TaylorDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, 53705, USA.
Randall J KimpleMcArdle Laboratory for Cancer Research, Madison, WI, 53705, USA.
David YangDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, 53705, USA.
Paul M HarariDepartment of Human Oncology, University of Wisconsin-Madison, Madison, WI, 53705, USA.
Paul F LambertMcArdle Laboratory for Cancer Research, Madison, WI, 53705, USA.
Ricardo V LloydDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, 53705, USA.
Rong HuDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, 53705, USA. rhu6@wisc.edu.

Funding

Visualizing EBV and HCMV DNA Dynamics During InfectionP01CA022443 · NCI · UNIVERSITY OF WISCONSIN-MADISON · PI Paul F. Lambert · 1985 to 2026
$53.1M
Project 3: Modulation of the head and neck tumor immune microenvironment by targeting the TAM family of receptorsP50CA278595 · NCI · UNIVERSITY OF WISCONSIN-MADISON · PI David J Beebe · 2022 to 2026
$12.5M
NCI NIH HHS P01 CA022443NCI NIH HHS P50 CA278595
6 · The paper itself

Abstract

backgroundHPV- associated squamous cell carcinoma (SCC) is uncommon in non-oropharynx sites and not well characterized. This study aims to investigate uncommon phenotypes of HPV-associated head and neck carcinoma, the prevalence and morphologic spectrum of HPV-associated SCC in the oral cavity, larynx and hypopharynx.

methodP16 immunostaining and HPV E6/7 in situ hybridization (ISH) were performed on tissue microarrays comprised of SCCs from different anatomic sites: oropharynx (n = 270), hypopharynx (n = 52), oral cavity (n = 95) and larynx (n = 123). Tumors were classified as HPV-associated based on a positive E6/7 ISH testing. RNA sequencing was performed on several selected cases.

result66% oropharynx SCCs (OPSCCs) were HPV-associated; all were p16/HPV testing concordant except one which was p16 negative. The p16-/HPV + OPSCC resembled similar gene expression signature with p16+/HPV + OPSCCs by transcriptome analysis. 6/95 (6%) oral cavity SCCs were HPV-associated, all from male patients and 5/6 (83%) arose from the floor of mouth. Morphologically, 3/6 (50%) showed keratinizing SCC and 5/6 (83%) demonstrated HPV-associated squamous dysplasia in adjacent mucosa. 1/123 (less than 1%) larynx SCCs and 0/52 hypopharynx SCCs were HPV-associated.

conclusionAlthough uncommon, p16 negative HPV-associated OPSCC can occur, emphasizing the importance of judicious HPV testing. The morphology of HPV-associated oral cavity SCCs may deviate from prototypic nonkeratinizing SCC, making them difficult to recognize. Presence of HPV-associated squamous dysplasia could serve as a morphologic clue.

Indexed as

Papillomavirus InfectionsPhenotypeSquamous Cell Carcinoma of Head and NeckAdultAgedAged, 80 and overFemaleHead and Neck NeoplasmsHuman Papillomavirus VirusesHumansMaleMiddle AgedE6/7HPVOral cavityOropharynxp16Squamous cell carcinoma

Identifiers

PMID39436498
PMCPMC11496466

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.