Evidence map›Paper›PMID 39436480›Full record

ArticleHuman cell2024

Interaction of SARS-CoV-2 Spike protein with ACE2 induces cortical actin modulation, including dephosphorylation of ERM proteins and reduction of cortical stiffness.

Thi Ly Do, Kouichi Tachibana, Norio Yamamoto, Kiyoshi Ando, Takaaki Isoda, Takanori Kihara

Abstract read
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In one paragraph

Article in Human cell, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Thi Ly DoDepartment of Life and Environment Engineering, Faculty of Environmental Engineering, The University of Kitakyushu, 1-1 Hibikino, Wakamatsu, Kitakyushu, Fukuoka, 808-0135, Japan.
Kouichi TachibanaDivision of Hematology and Oncology, Department of Internal Medicine, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
Norio YamamotoDepartment of Microbiology, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
Kiyoshi AndoDivision of Hematology and Oncology, Department of Internal Medicine, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan.
Takaaki IsodaDepartment of Life and Environment Engineering, Faculty of Environmental Engineering, The University of Kitakyushu, 1-1 Hibikino, Wakamatsu, Kitakyushu, Fukuoka, 808-0135, Japan.
Takanori KiharaDepartment of Life and Environment Engineering, Faculty of Environmental Engineering, The University of Kitakyushu, 1-1 Hibikino, Wakamatsu, Kitakyushu, Fukuoka, 808-0135, Japan. tkihara@kitakyu-u.ac.jp.ORCID http://orcid.org/0000-0002-5052-6380

Funding

Adaptable and Seamless Technology Transfer Program through Target-Driven R and D JPMJTR21U4Institute of Environmental Science and Technology, The University of Kitakyushu Grant for Young ScientistsJapan Society for the Promotion of Science JP21K04797Japan Society for the Promotion of Science JP24K15731Ministry of Education, Culture, Sports, Science and Technology JPMXP0621467946
6 · The paper itself

Abstract

Cell surface cortical actin is a regulatory target for viral infection. We aimed to investigate the effect of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection on host cell cortical stiffness, an indicator of cortical actin structure. The receptor-binding domain (RBD) of SARS-CoV-2 Spike (S) protein induced a reduction in cortical stiffness in ACE2-expressing cells. The interaction of RBD with ACE2 caused the inactivation of Ezrin/Radixin/Moesin (ERM) proteins. We further investigated the effects of the RBD of SARS-CoV-2 Omicron variants, BA.1 and BA.5. These RBDs influenced cortical stiffness depending on their affinity for ACE2. Our study provides the first evidence that the interaction of the SARS-CoV-2 S protein with ACE2 induces mechanobiological signals and attenuates the cortical actin.

Indexed as

ActinsAngiotensin-Converting Enzyme 2Cytoskeletal ProteinsMembrane ProteinsMicrofilament ProteinsSpike Glycoprotein, CoronavirusCOVID-19EzrinHumansPhosphorylationProtein BindingProtein DomainsSARS-CoV-2ACE2 protein, humanActinsAngiotensin-Converting Enzyme 2Cytoskeletal ProteinsEzrinMembrane ProteinsMicrofilament ProteinsmoesinradixinSpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2ACE2Cell stiffnessCortical actinERMSARS-CoV-2

Identifiers

PMID39436480

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.