ArticleNature plants2024
Total biosynthesis of the medicinal triterpenoid saponin astragalosides.
Article in Nature plants, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Complete biosynthesis of the clinical agent escin Ia.Nature communications · 2026Article
- Chromosomal scale genome assembly of medicinal plant Sophora tonkinensis.BMC genomics · 2026Article
- The Oplopanax elatus genome reveals dammaradienol synthase evolution enabling reconstruction of RK type ginsenosides biosynthesis.Nature communications · 2026Article
- Insights into the Cytochrome P450 Monooxygenase Superfamily inMolecules (Basel, Switzerland) · 2026Article
- Analysis of BpbHLH Gene Family Responsive to MeJA Signalling in Betula platyphylla Suk. and Functional Mechanisms of BpbHLH42/44 in Genetic Improvement and Triterpenoid Biosynthesis.Plant biotechnology journal · 2026Article
- Elucidating the mechanisms underlying differential anthocyanin biosynthesis and its link to stem color and root isoflavonoid levels inHorticulture research · 2026Article
- Molecular Networking-Guided Phytochemical Profiling and Anti-Inflammatory Evaluation of Honglanqi, an Underutilized Commercial Specification of Astragali Radix.Plants (Basel, Switzerland) · 2026Article
- Exploring specialized metabolic pathways in medicinal plants with single-cell and spatial omics.Acta pharmaceutica Sinica. B · 2026Review
- Valorization of ligustrum species: biosynthesis, metabolic engineering, and pharmacology of bioactive compounds.Bioresources and bioprocessing · 2026Review
- Biomanufacturing of Natural Bioactive Compounds.Molecules (Basel, Switzerland) · 2026Article
- Plastid 2-oxoglutarate-dependent dioxygenases mediate stereoselective C14β-hydroxylation in cardenolide biosynthesis.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- A review of glycosylation diversity and biosynthetic advances of steroidal saponins inFrontiers in plant science · 2026Review
- Herbgenomics: Unraveling natural product biosynthesis in traditional Chinese medicine.Chinese herbal medicines · 2026Article
- A Modular Chemoenzymatic Cascade Simplifies Divergent Synthesis of Natural and Unnatural Benzylisoquinoline Alkaloids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The effects of traditional Chinese botanical medicine on membranous nephropathy.Frontiers in pharmacology · 2026Review
- Structural classification, biosynthesis, metabolic engineering and ecological functions for the terpenes produced in tobacco.Frontiers in plant science · 2026Review
- AmDB: functional genomics database of Astragalus membranaceus.BMC genomics · 2025Article
- Production of cycloastragenol in metabolically engineered yeast.Engineering microbiology · 2025Article
- Biomimetic membrane-coated nanoparticles for targeted synergistic therapy of homocysteine-induced atherosclerosis: Dual modulation of cholesterol efflux and reactive oxygen species scavenging.Materials today. Bio · 2025Article
- Dihydroartemisinic acid dehydrogenase-mediated alternative route for artemisinin biosynthesis.Nature communications · 2025Article
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Authors and funding
12 authors.
Funding
Abstract
Astragalus membranaceus has been used in traditional Chinese medicine for over 2,000 years. Its major active triterpenoid saponins, astragalosides, have attracted great attention due to their multiple health benefits and applications in medicine. Despite this, the biosynthetic machinery for astragalosides remains enigmatic. Here a chromosome-level genome assembly of A. membranaceus was generated. The identification of two tailoring enzymes required for astragaloside biosynthesis enabled the discovery of a triterpenoid biosynthetic gene cluster, leading to elucidation of the complete astragaloside biosynthetic pathway. This pathway is characterized by a sequence of selective hydroxylation, epoxidation and glycosylation reactions, which are mediated by three cytochrome P450s, one 2-oxoglutarate-dependent dioxygenase and two glycosyltransferases. Reconstitution of this biosynthetic machinery in Nicotiana benthamiana allowed for heterologous production of astragaloside IV. These findings build a solid foundation for addressing the sourcing issues associated with astragalosides and broaden our understanding of the diversity of terpene biosynthetic gene clusters.
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