Evidence map›Paper›PMID 39433683›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

In Vitro and In Vivo Methods for Studying Retinal Ganglion Cell Survival and Optic Nerve Regeneration.

Yuqin Yin, Larry I Benowitz

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Yuqin YinDepartment of Neurosurgery and F.M. Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA, USA. yuqin.yin@childrens.harvard.edu.
Larry I BenowitzDepartment of Neurosurgery and F.M. Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA, USA.

Funding

MOLECULAR BASES OF NEURONAL CONNECTIVITYR01EY005690 · NEI · MC LEAN HOSPITAL (BELMONT, MA) · PI BENOWITZ, LARRY IRA · 1985 to 2012
$4.7M
Novel growth factors and regenerative RGC subtypes for optic nerve regenerationR21EY033550 · NEI · BOSTON CHILDREN'S HOSPITAL · PI YIN, YUQIN · 2023 to 2024
$487k
NEI NIH HHS R01 EY005690NEI NIH HHS R21 EY033550
6 · The paper itself

Abstract

Glaucoma is marked by a progressive degeneration of the optic nerve and delayed loss of retinal ganglion cells (RGCs), the projection neurons of the eye. Because RGCs are not replaced and because surviving RGCs cannot regenerate their axons, the visual loss in glaucoma is largely irreversible. Here we describe methods to evaluate treatments that may be beneficial for treating glaucoma using in vitro cell culture models (immunopanning to isolate neonatal RGCs, dissociated mature retinal neurons, retinal explants) and in vivo models that test potential treatments or investigate underlying molecular mechanisms in an intact system. Potentially, the use of these models can help investigators continue to improve treatments to preserve RGCs and restore visual function in patients with glaucoma.

Indexed as

Cell SurvivalNerve RegenerationOptic NerveRetinal Ganglion CellsAnimalsCell Culture TechniquesCells, CulturedGlaucomaMiceRatsAxon regenerationCell cultureCell signaling mechanismsExplantsGlaucomaOptic nerveQuantitative methodsRetinaRetinal ganglion cellsTrophic factors

Identifiers

PMID39433683

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.