Evidence map›Paper›PMID 39433681›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

A Comprehensive Protocol for Microbead-Induced Ocular Hypertension in Mice.

M Reza Bahrani Fard, Nina Sara Fraticelli-Guzmán, Jessica Chan, A Thomas Read, Andrew Feola, W Daniel Stamer, Simon W M John, C Ross Ethier, Rebecca M Sappington

Erratum issuedAbstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

M Reza Bahrani FardGeorge W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Nina Sara Fraticelli-GuzmánGeorge W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Jessica ChanDepartment of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, USA.
A Thomas ReadWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology/Emory University, Atlanta, GA, USA.
Andrew FeolaWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology/Emory University, Atlanta, GA, USA.
W Daniel StamerDepartment of Ophthalmology, Duke University, Durham, NC, USA.
Simon W M JohnDepartment of Ophthalmology, Columbia University Irving Medical Center, New York, NY, USA.
C Ross EthierWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology/Emory University, Atlanta, GA, USA. ross.ethier@bme.gatech.edu.
Rebecca M SappingtonDepartment of Biochemistry, Wake Forest University School of Medicine, Winston-Salem, NC, USA. rsapping@wakehealth.edu.

Funding

Transcriptional and Biomechanical Analysis of Segmental Outflow in GlaucomaR01EY031710 · NEI · GEORGIA INSTITUTE OF TECHNOLOGY · PI ETHIER, C ROSS, GUPTA, DIVAKAR · 2020 to 2023
$2.4M
NEI NIH HHS R01 EY031710
6 · The paper itself

Abstract

Glaucoma is a common optic neuropathy characterized by degeneration of retinal ganglion cells (RGCs). Elevated intraocular pressure (IOP), that is, ocular hypertension, is the primary modifiable risk factor for glaucoma and the primary characteristic of most preclinical glaucoma models. Extensive genotype and phenotype diversity at relatively low cost and high accessibility makes laboratory mice an excellent preclinical model for glaucoma. The microbead occlusion model was introduced in 2010 as an inducible model of ocular hypertension in mice and is now one of the most extensively utilized models of rodent glaucoma. Subsequent modifications of the microbead model increased the magnitude and duration of IOP elevation, primarily through modification of injection materials. Despite its popularity, accessibility of the model is hindered by procedural consistency between users. Here we outline an updated and comprehensive protocol for execution of the microbead model that is focused on improving surgical and outcome measure consistency and on enabling single experimenter execution.

Indexed as

Disease Models, AnimalGlaucomaIntraocular PressureOcular HypertensionAnimalsMiceRetinal Ganglion CellsGlaucomaIntraocular pressureMicrobeadMouse modelOcular hypertensionRodent

Identifiers

PMID39433681

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.