Evidence map›Paper›PMID 39433671›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Human Corneal Rim Perfusion Culture.

Weiming Mao

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

Weiming MaoDepartments of Ophthalmology, Biochemistry & Molecular Biology, and Pharmacology & Toxicology, Eugene and Marilyn Glick Eye Institute, Stark Neurosciences Research Institute, Indiana University School of Medicine, Indianapolis, IN, USA. weimmao@iu.edu.

Funding

The role of Wnt signaling in treating glucocorticoid-induced glaucomaR01EY031700 · NEI · INDIANA UNIVERSITY INDIANAPOLIS · PI MAO, WEIMING · 2021 to 2025
$2.5M
Cross-talk between TGF-beta and Wnt pathways in the trabecular meshworkR01EY026962 · NEI · UNIVERSITY OF NORTH TEXAS HLTH SCI CTR · PI MAO, WEIMING · 2017 to 2021
$1.9M
The role of distal aqueous humor outflow tissue in glucocorticoid-induced glaucomaR21EY033929 · NEI · INDIANA UNIVERSITY INDIANAPOLIS · PI MAO, WEIMING · 2023 to 2024
$436k
Identification of genes responsible for glucocorticoid-induced glaucomaR21EY023048 · NEI · UNIVERSITY OF NORTH TEXAS HLTH SCI CTR · PI MAO, WEIMING · 2013 to 2014
$388k
NEI NIH HHS R01 EY026962NEI NIH HHS R01 EY031700NEI NIH HHS R21 EY023048NEI NIH HHS R21 EY033929
6 · The paper itself

Abstract

Human anterior segment perfusion cultures are frequently used for trabecular meshwork research. However, this model requires the use of whole eye globes which are expensive. Here, we describe a method using human corneal rims as an alternative to anterior segments for perfusion culture. In this method, the human corneal rim is glued to a three-dimensional-printed perfusion plates instead of using mechanical compression in traditional perfusion culture setup. The corneal rim and perfusion plate are placed onto a special holder for perfusion culture. This method is affordable and easy to set up. The instruments for traditional perfusion culture studies can be used for this model without modification.

Indexed as

CorneaPerfusionCell Culture TechniquesHumansOrgan Culture TechniquesPrinting, Three-DimensionalTrabecular MeshworkCorneal rim perfusion cultureIntraocular pressureOutflow facility

Identifiers

PMID39433671

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.