Evidence map›Paper›PMID 39433669›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Human Anterior Segment Perfusion Organ Culture.

Uttio Roy Chowdhury, Michael P Fautsch

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Uttio Roy ChowdhuryDepartment of Ophthalmology, Mayo Clinic, Rochester, MN, USA.
Michael P FautschDepartment of Ophthalmology, Mayo Clinic, Rochester, MN, USA. fautsch.michael@mayo.edu.

Funding

Intraocular pressure regulation via ATP-sensitive potassium channelsR01EY021727 · NEI · MAYO CLINIC ROCHESTER · PI FAUTSCH, MICHAEL P. · 2011 to 2021
$4.1M
NEI NIH HHS R01 EY021727
6 · The paper itself

Abstract

The human anterior segment perfusion organ culture is an ex vivo model system for studying the human conventional outflow pathway with reference to pressure regulation. In this model, anterior segments dissected from human donor eyes can be fixed to a modified petri dish and perfused with media along with various study agents at the physiological flow rate of 2.5 μL/min. The model mimics the one-way flow of aqueous humor in human eyes and can be used to evaluate the effects of various drugs on eye pressure in real time. Using this model, cells and tissues of the anterior segment can be maintained for up to 28 days, enabling histological and molecular evaluations.

Indexed as

Anterior Eye SegmentOrgan Culture TechniquesPerfusionAqueous HumorHumansAnterior segment perfusion cultureConventional outflowDistal outflowEx vivo eye culturePerfusion organ cultureTrabecular meshwork

Identifiers

PMID39433669

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.