Evidence map›Paper›PMID 39433663›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Three-Dimensional Extracellular Matrix Protein Hydrogels for Human Trabecular Meshwork Cell Studies.

Ayushi Singh, Rajanya Ghosh, Haiyan Li, Michael P Geiss, Hannah Yoo, Ana N Strat, Preethi S Ganapathy, Samuel Herberg

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Considerations and implications of currentFrontiers in cell and developmental biology · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ayushi Singh *Department of Ophthalmology and Visual Sciences, SUNY Upstate Medical University, Syracuse, NY, USA.
Rajanya Ghosh *Department of Ophthalmology and Visual Sciences, SUNY Upstate Medical University, Syracuse, NY, USA.
Haiyan Li *Department of Ophthalmology and Visual Sciences, SUNY Upstate Medical University, Syracuse, NY, USA.
Michael P GeissDepartment of Ophthalmology and Visual Sciences, SUNY Upstate Medical University, Syracuse, NY, USA.
Hannah YooDepartment of Ophthalmology and Visual Sciences, SUNY Upstate Medical University, Syracuse, NY, USA.
Ana N StratDepartment of Ophthalmology and Visual Sciences, SUNY Upstate Medical University, Syracuse, NY, USA.
Preethi S GanapathyDepartment of Ophthalmology and Visual Sciences, SUNY Upstate Medical University, Syracuse, NY, USA.
Samuel HerbergDepartment of Ophthalmology and Visual Sciences, SUNY Upstate Medical University, Syracuse, NY, USA. herbergs@upstate.edu.

Funding

Role of cellular memory in glaucoma.R01EY034096 · NEI · UPSTATE MEDICAL UNIVERSITY · PI Samuel Herberg · 2022 to 2026
$2.0M
Investigating mechanosensation in the glaucomatous optic nerve headK08EY031755 · NEI · UPSTATE MEDICAL UNIVERSITY · PI GANAPATHY, PREETHI · 2021 to 2025
$1.1M
TM/SC-interface-on-a-chip for mechanistic studies of outflow regulation.R21EY036189 · NEI · UPSTATE MEDICAL UNIVERSITY · PI HERBERG, SAMUEL · 2024 to 2025
$439k
NEI NIH HHS K08 EY031755NEI NIH HHS R01 EY034096NEI NIH HHS R21 EY036189
6 · The paper itself

Abstract

The extracellular microenvironment plays a crucial role in regulating a wide range of cell behaviors. Biopolymer hydrogels are ideally suited to present a realistic three-dimensional extracellular milieu to cells in vitro. Here, we describe the fabrication and use of soft tissue-mimetic extracellular matrix protein hydrogels for investigations of human trabecular meshwork cell biology.

Indexed as

Extracellular Matrix ProteinsHydrogelsTrabecular MeshworkCell Culture TechniquesCells, CulturedExtracellular MatrixHumansExtracellular Matrix ProteinsHydrogelsBioengineeringBiopolymersGlaucomaOutflow tractPhoto-crosslinking

Identifiers

PMID39433663
PMCPMC11571287

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.