Evidence map›Paper›PMID 39428988›Full record

ArticleBiology open2024

Glutaraldehyde-enhanced autofluorescence as a general tool for 3D morphological imaging.

Miika Niemeläinen, Anna-Mari Haapanen-Saaristo, Leena M Koskinen, Josef Gullmets, Emilia Peuhu, Annika Meinander, Sara Calhim, Ilkka Paatero

Abstract read
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In one paragraph

Article in Biology open, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Miika NiemeläinenTurku Bioscience Centre, University of Turku and Åbo Akademi University, Turku FI-20520, Finland.
Anna-Mari Haapanen-SaaristoTurku Bioscience Centre, University of Turku and Åbo Akademi University, Turku FI-20520, Finland.
Leena M KoskinenInstitute of Biomedicine, Cancer Laboratory FICAN west , University of Turku, Turku FI-20520, Finland.
Josef GullmetsFaculty of Science and Engineering, Cell Biology, Åbo Akademi University, BioCity, Turku FI-20520, Finland.
Emilia PeuhuInstitute of Biomedicine, Cancer Laboratory FICAN west , University of Turku, Turku FI-20520, Finland.
Annika MeinanderFaculty of Science and Engineering, Cell Biology, Åbo Akademi University, BioCity, Turku FI-20520, Finland.ORCID 0000-0002-3878-2293
Sara CalhimDepartment of Biological and Environmental Science, University of Jyväskylä, Jyväskylä FI-40014, Finland.
Ilkka PaateroTurku Bioscience Centre, University of Turku and Åbo Akademi University, Turku FI-20520, Finland.ORCID 0000-0001-5926-2396

Funding

Åbo Akademi University Foundation. Sigrid Jusélius Foundation. Academy of Finland 323096Business Finland 1718/31/2021FinnFoto 20220515FinnFOTO 20220515Research Council of Finland 323096The Hospital District of Southwest Finland 11083Turku Doctoral Program of Molecular MedicineUniversity of Turku: Turun YliopistoVarsinais-Suomen Sairaanhoitopiiri 11083
6 · The paper itself

Abstract

Routine histochemical techniques are capable of producing vast amount of information from diverse sample types, but these techniques are limited in their ability to generate 3D information. Autofluorescence imaging can be used to analyse samples in 3D but it suffers from weak/low signal intensities. Here, we describe a simple chemical treatment with glutaraldehyde to enhance autofluorescence for 3D fluorescence imaging and to generate detailed morphological images on whole-mount samples. This methodology is straightforward and cost-effective to implement, suitable for a wide range of organisms and sample types. Furthermore, it can be readily integrated with standard confocal and fluorescence microscopes for analysis. This approach has the potential to facilitate the analysis of biological 3D structures and research in developmental biology, including studies on model and non-model organisms.

Indexed as

GlutaralImaging, Three-DimensionalOptical ImagingAnimalsHumansMicroscopy, ConfocalMicroscopy, FluorescenceGlutaral3D imagingAutofluorescenceDevelopmental biologyMicroscopyModel organismNon-model organism

Identifiers

PMID39428988
PMCPMC11583915

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.