ArticleFEBS open bio2025
Revealing protein structures: crystallization of protein-ligand complexes - co-crystallization and crystal soaking.
Article in FEBS open bio, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- Developments and challenges in hit progression within fragment-based drug discovery.Nature communications · 2026Review
- A step further in our commitment to support early career researchers (ECRs).FEBS open bio · 2026Article
- Other Techniques.Advances in biochemical engineering/biotechnology · 2026Review
- Automated gradient equilibration of macromolecular crystals to new solution conditions.Acta crystallographica. Section F, Structural biology communications · 2025Article
- Revisiting Proteus 2.0: Two Decades of Pioneering Lectin Crystallography at BioMol-Lab in Northeast Brazil.ACS omega · 2025Review
- Improving experimental reproducibility through comprehensive research protocols.FEBS open bio · 2025Article
- Ascertaining a Structural Basis in Drug Discovery and Development.Journal of medicinal chemistry · 2025Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Protein crystallogenesis represents a key step in X-ray crystallography studies that employ co-crystallization and ligand soaking for investigating ligand binding to proteins. Co-crystallization is a method that enables the precise determination of binding positions, although it necessitates a significant degree of optimization. The utilization of microseeding can facilitate a reduction in sample requirements and accelerate the co-crystallization process. Ligand soaking is the preferred method due to its simplicity; however, it requires careful control of soaking conditions to ensure the successful integration of the ligands. This research protocol details the procedures for co-crystallization and soaking to achieve protein-ligand complex formation, which is essential for advancing drug discovery. Additionally, a simple protocol for demonstrating soaking for educational purposes is described.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.