Evidence map›Paper›PMID 39425817›Full record

ArticleMolecular biology reports2024

Identification and analysis of the key genes for Escherichia coli heterologous protein expression by transcriptomic profiling.

Anxiang Chen, Yuguo Dong, Huaigu Jiang, Shengli Yang, Jian Zhang, Dongzhi Wei

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

6 authors.

Anxiang Chen *State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.
Yuguo Dong *State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.
Huaigu JiangState Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.
Shengli YangState Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.
Jian ZhangState Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China. zhangjian0020@ecust.edu.cn.
Dongzhi WeiState Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.

Funding

The National Key Research and Development Program of China 2022YFA0912200
6 · The paper itself

Abstract

backgroundEscherichia coli is a frequently used host for heterologous protein expression, but its expression efficiency is hindered by several limitations, such as formation of inclusion bodies and proteolytic degradation. METHODS AND

resultsIn this study, we employed high-density fermentation of heterologous protein production in a 5-L bioreactor, resulting in a yield 2.25 times higher than that of the control group. Transcriptional analysis was conducted at three time points after induction for 0 h, 4 h, and 12 h, revealing 420, 301, and 570 upregulated differentially expressed genes, as well as 424, 202, and 525 downregulated genes, respectively. By conducting enrichment analysis, we constructed strains that relieved without iron limitation, exhibiting a 36% increase in biomass and a 32% increase in protein expression. Furthermore, no overflow metabolism of acetic acid was detected during the protein expression process when utilizing chemostat culture, which indicated that the utilization efficiency of glucose was significantly enhanced without iron limitation.

conclusionsThis study presents a novel approach to better comprehend the mechanism of high-yield production of heterologous proteins in Escherichia coli.

Indexed as

BioreactorsEscherichia coliFermentationGene Expression ProfilingGene Expression Regulation, BacterialBiomassEscherichia coli ProteinsGlucoseIronRecombinant ProteinsTranscriptomeEscherichia coli ProteinsGlucoseIronRecombinant ProteinsEscherichia coliHigh density fermentationIron restrictionProtein expressionTranscriptome analysis

Identifiers

PMID39425817

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