Evidence map›Paper›PMID 39425195›Full record

ArticleBMC veterinary research2024

Challenges in diagnosing bovine tuberculosis through surveillance and characterization of Mycobacterium species in slaughtered cattle in Kolkata.

Molla Zakirul Haque, Chanchal Guha, Ayan Mukherjee, Sukhen Samanta, Partha Sarathi Jana, Ujjwal Biswas, Sangeeta Mandal, Santanu Pal, Manigandan Venkatesan, Joy Sarojini Michael and 4 more

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Article in BMC veterinary research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

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0cells of the map it votes in
3citing papers in PubMed
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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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3 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

14 authors.

Molla Zakirul HaqueWest Bengal University of Animal and Fishery Sciences, 37 & 68, Kshudiram Bose Sarani, Kolkata, West Bengal, 700 037, India.
Chanchal GuhaWest Bengal University of Animal and Fishery Sciences, 37 & 68, Kshudiram Bose Sarani, Kolkata, West Bengal, 700 037, India.
Ayan MukherjeeWest Bengal University of Animal and Fishery Sciences, 37 & 68, Kshudiram Bose Sarani, Kolkata, West Bengal, 700 037, India.
Sukhen SamantaICAR-Indian Veterinary Research Institute, Eastern Regional Station, 37, Belgachia Road, Kolkata, West Bengal, 700 037, India.
Partha Sarathi JanaWest Bengal University of Animal and Fishery Sciences, 37 & 68, Kshudiram Bose Sarani, Kolkata, West Bengal, 700 037, India.
Ujjwal BiswasWest Bengal University of Animal and Fishery Sciences, 37 & 68, Kshudiram Bose Sarani, Kolkata, West Bengal, 700 037, India.
Sangeeta MandalWest Bengal University of Animal and Fishery Sciences, 37 & 68, Kshudiram Bose Sarani, Kolkata, West Bengal, 700 037, India.
Santanu PalICAR-Indian Veterinary Research Institute, Eastern Regional Station, 37, Belgachia Road, Kolkata, West Bengal, 700 037, India.
Manigandan VenkatesanChristian Medical College, Vellore, Tamil Nadu, 632 004, India.
Joy Sarojini MichaelChristian Medical College, Vellore, Tamil Nadu, 632 004, India.
Pramod Kumar NandaICAR-Indian Veterinary Research Institute, Eastern Regional Station, 37, Belgachia Road, Kolkata, West Bengal, 700 037, India.
Samiran BandyopadhyayICAR-Indian Veterinary Research Institute, Eastern Regional Station, 37, Belgachia Road, Kolkata, West Bengal, 700 037, India.
Arun K DasICAR-Indian Veterinary Research Institute, Eastern Regional Station, 37, Belgachia Road, Kolkata, West Bengal, 700 037, India.
Premanshu DandapatICAR-Indian Veterinary Research Institute, Eastern Regional Station, 37, Belgachia Road, Kolkata, West Bengal, 700 037, India. pdandapat@gmail.com.

Funding

Department of Biotechnology, Ministry of Science and Technology, Government of India BT/ADV/Bovine Tuberculosis/2018
6 · The paper itself

Abstract

backgroundTuberculosis in cattle is caused by Mycobacterium tuberculosis complex (MTBC) species. Apart from MTBC, different Nontuberculous Mycobacteria (NTM) species have also been isolated from cattle. The presence of NTM infection in bovines makes the diagnosis of bovine tuberculosis (bTB) a cumbersome task. Therefore, a cross sectional study was conducted to isolate and characterize different Mycobacterium spp. from a slaughterhouse situated in Kolkata, a city in the eastern part of India.

resultsOut of 258 morbid samples, 98 isolates were found to be positive for bacterial growth, and 35% (n = 34) were positive for Mycobacterium. 94% of Mycobacterial cultural isolates were NTM (n = 32), and the rest (n = 2) were found to be MTBC. Species-level identification of the isolates by hsp65 sequencing revealed that out of 32 isolates, 24 were M. fortuitum, three were M. abscessus, two each were M. chelonae and M. parascrofulaceum, and one was M. novocastrense. A phylogenetic tree with partial hsp65 gene sequences was also constructed to determine the relatedness of the unknown isolates to the reference strains.

conclusionBoth NTM species and MTBCs were identified from TB-like lesions in cattle that were slaughtered at the Kolkata abattoir. This discovery may indicate that NTM contributes to the development of lesions in cattle. Also, we recommend implication of more specific diagnostic tests for bTB.

Indexed as

AbattoirsMycobacteriumPhylogenyTuberculosis, BovineAnimalsBacterial ProteinsCattleChaperonin 60Cross-Sectional StudiesIndiaNontuberculous MycobacteriaBacterial ProteinsChaperonin 60AbattoirDuplex PCRhsp65Nontuberculous MycobacteriaZoonotic bovine tuberculosis

Identifiers

PMID39425195
PMCPMC11488179

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.