Evidence map›Paper›PMID 39423819›Full record

ArticleDevelopmental cell2025

Outer mitochondrial membrane E3 Ub ligase MARCH5 controls de novo peroxisome biogenesis.

Nicolas Verhoeven, Yumiko Oshima, Etienne Cartier, Claudia Christiane Bippes, Albert Neutzner, Liron Boyman, Mariusz Karbowski

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental cell, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Peroxisome Dysfunction and Steatotic Liver Disease.International journal of molecular sciences · 2025
    Review
  6. Mitochondrial metabolism and cancer therapeutic innovation.Signal transduction and targeted therapy · 2025
    Review
  7. Review
  8. Review
  9. Review
  10. Article
  11. Article
  12. What Peroxisomes (Don't) do to Mitochondria.Contact (Thousand Oaks (Ventura County, Calif.))
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Nicolas VerhoevenCenter for Biomedical Engineering and Technology, University Hospital Basel, University of Basel, Basel, Switzerland; Department of Biochemistry and Molecular Biology, University Hospital Basel, University of Basel, Basel, Switzerland.
Yumiko OshimaCenter for Biomedical Engineering and Technology, University Hospital Basel, University of Basel, Basel, Switzerland; Department of Biochemistry and Molecular Biology, University Hospital Basel, University of Basel, Basel, Switzerland.
Etienne CartierCenter for Biomedical Engineering and Technology, University Hospital Basel, University of Basel, Basel, Switzerland; Department of Biochemistry and Molecular Biology, University Hospital Basel, University of Basel, Basel, Switzerland.
Claudia Christiane BippesDepartment of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland.
Albert NeutznerDepartment of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland.
Liron BoymanCenter for Biomedical Engineering and Technology, University Hospital Basel, University of Basel, Basel, Switzerland; Department of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA; University of Maryland Marlene and Stewart Greenebaum Comprehensive Cancer Center, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
Mariusz KarbowskiCenter for Biomedical Engineering and Technology, University Hospital Basel, University of Basel, Basel, Switzerland; Department of Biochemistry and Molecular Biology, University Hospital Basel, University of Basel, Basel, Switzerland; Department of Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA. Electronic address: mkarbowski@som.umaryland.edu.

Funding

Ubiquitin-dependent mitochondrial quality controlR01GM129584 · NIGMS · UNIVERSITY OF MARYLAND BALTIMORE · PI KARBOWSKI, MARIUSZ · 2019 to 2022
$1.3M
NIGMS NIH HHS R01 GM129584
6 · The paper itself

Abstract

We report that the outer mitochondrial membrane (OMM)-associated E3 Ub ligase MARCH5 is vital for generating mitochondria-derived pre-peroxisomes. In human immortalized cells, MARCH5 knockout leads to the accumulation of immature peroxisomes, reduced fatty-acid-induced peroxisomal biogenesis, and abnormal peroxisome biogenesis in MARCH5/Pex14 and MARCH5/Pex3 dko cells. Upon fatty-acid-induced peroxisomal biogenesis, MARCH5 redistributes to peroxisomes, and ubiquitination activity-deficient mutants of MARCH5 accumulate on peroxisomes containing high levels of the OMM protein Tom20 (mitochondria-derived pre-peroxisomes). Similarly, depletion of peroxisome biogenesis factor Pex14 leads to the accumulation of MARCH5- and Tom20-positive pre-peroxisomes, whereas no peroxisomes are detected in MARCH5/Pex14 dko cells. Inconsistent with MARCH5 merely acting as a quality factor, mitochondrial decline is not evident in tested models. Furthermore, reduced expression of peroxisomal proteins is detected in MARCH5

Indexed as

Membrane ProteinsMitochondriaMitochondrial MembranesPeroxinsPeroxisomesUbiquitinationUbiquitin-Protein LigasesFatty AcidsHumansLipoproteinsOrganelle BiogenesisPeroxisome-Targeting Signal 1 ReceptorReceptors, Cytoplasmic and NuclearRepressor ProteinsFatty AcidsLipoproteinsMARCHF5 protein, humanMembrane ProteinsPeroxinsPeroxisome-Targeting Signal 1 ReceptorPEX14 protein, humanPex3 protein, humanReceptors, Cytoplasmic and NuclearRepressor ProteinsUbiquitin-Protein LigasesbiogenesisMARCH5metabolic adaptationmitochondriamitochondria-derived pre-peroxisomesperoxisomesPex14Pex3Ub E3 ligase

Identifiers

PMID39423819
PMCPMC11706706

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.