ArticleSTAR protocols2024
Protocol for the generation of single-nuclei RNA-seq libraries and quantification of heterogeneous cell types activated during social interaction.
Article in STAR protocols, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Blood IL-6 is a critical trigger of depressive symptoms in a mouse model for human atopic dermatitis.Translational psychiatry · 2026Article
- Stress resilience is associated with transcriptional remodeling in the VTA.Cell reports · 2026Article
- Stress resilience is associated with transcriptional remodeling in the VTA.bioRxiv : the preprint server for biology · 2025Article
- Impaired Spatiotemporal Encoding of Social Behavior and Anxiety in the Prefrontal Cortex of Mice Lacking ASD-Risk GenebioRxiv : the preprint server for biology · 2025Article
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2 authors.
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Abstract
Quantifying immediate early gene expression as a marker of cellular activity in single-nuclei RNA sequencing data allows for the identification of neurons involved in specific behaviors. Here, we present a protocol for generating single-nuclei libraries from the mouse brain and identifying active cell populations following social interactions. We describe steps for the dissection, preparation, and analysis of the prefrontal cortex, hippocampus, and cerebellum. This protocol has the potential to be modified for any brain region or behavior of interest. For complete details on the use and execution of this protocol, please refer to Walker and Frost.
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