Evidence map›Paper›PMID 39422788›Full record

ArticleMolecular biology reports2024

MicroRNA expression profiles in plasma exosomes of late pregnant giant pandas.

Meiling Cheng, Yingmin Zhou, Qian Wang, Bo Luo, Yanwu Lai, Jianbin Cheng, Xiuyue Zhang, Yan Huang, Desheng Li

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In one paragraph

Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Meiling ChengKey Laboratory of State Forestry and Grassland Administration on the Giant Panda, China Conservation and Research Center for the Giant Panda, Chengdu, 610051, China.
Yingmin ZhouKey Laboratory of State Forestry and Grassland Administration on the Giant Panda, China Conservation and Research Center for the Giant Panda, Chengdu, 610051, China. pandac20@126.com.
Qian WangKey Laboratory of State Forestry and Grassland Administration on the Giant Panda, China Conservation and Research Center for the Giant Panda, Chengdu, 610051, China.
Bo LuoKey Laboratory of State Forestry and Grassland Administration on the Giant Panda, China Conservation and Research Center for the Giant Panda, Chengdu, 610051, China.
Yanwu LaiKey Laboratory of State Forestry and Grassland Administration on the Giant Panda, China Conservation and Research Center for the Giant Panda, Chengdu, 610051, China.
Jianbin ChengKey Laboratory of State Forestry and Grassland Administration on the Giant Panda, China Conservation and Research Center for the Giant Panda, Chengdu, 610051, China.
Xiuyue ZhangKey Laboratory of Bioresources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, 610064, China.
Yan HuangKey Laboratory of State Forestry and Grassland Administration on the Giant Panda, China Conservation and Research Center for the Giant Panda, Chengdu, 610051, China.
Desheng LiKey Laboratory of State Forestry and Grassland Administration on the Giant Panda, China Conservation and Research Center for the Giant Panda, Chengdu, 610051, China.

Funding

China Conservation and Research Center for the Giant Panda CCRCGP222318China Conservation and Research Center for the Giant Panda CCRCGP222320
6 · The paper itself

Abstract

backgroundMicroRNAs can regulate various biological functions including cell proliferation, differentiation, embryo formation, and implantation. The giant panda exhibits embryonic diapause, with embryo development resuming in late pregnancy. However, the changes in microRNAs during late pregnancy remain poorly understand. METHODS AND

resultsAfter mating, plasma samples were collected on day 40 of early pregnancy (EP; n = 3) and 30 days before delivery of late pregnancy (LP; n = 3). Following microRNAs screening, a total of 120 microRNAs were detected in the plasma exosomes of pregnant pandas. Nine differentially expressed microRNAs (DEmicroRNAs) were identified in LP compared to EP, including three that were upregulated and six that were downregulated. Notably, miR-25b and miR-47 were significantly downregulated in LP group. All DEmicroRNAs were predicted to target a total of 2,675 genes. Pathway enrichment analysis of these target genes revealed significant enrichment in the MAPK and Rap1 signaling pathways, which are closely related to cell proliferation, differentiation, and cell-cell and cell-matrix interactions. Analysis of protein-protein interaction networks showed that most of the hub genes (five out of eight), including Fgfr1, Fgf2, Fgf18, Erbb4, and Kras within the MAPK and Rap1 pathways are associated with the cell proliferation and differentiation. Significantly, Erbb4 was regulated by significantly differentially expressed miRNA-47.

conclusionsWe suggest that plasma exosomal microRNAs are involved in cell proliferation and differentiation during embryonic development by regulating key hub genes within MAPK and Rap1 pathways. These findings provided new insights into the development of giant panda embryos.

Indexed as

ExosomesMicroRNAsUrsidaeAnimalsCell ProliferationEmbryonic DevelopmentFemaleGene Expression ProfilingPregnancyProtein Interaction MapsSignal TransductionMicroRNAsGiant pandasLate pregnancyMicroRNAsPlasma exosomes

Identifiers

PMID39422788

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