Evidence map›Paper›PMID 39422499›Full record

ArticleAnalytical chemistry2024

Detection of >400 Cluster of Differentiation Biomarkers and Pathway Proteins in Single Immune Cells by Cyclic Multiplex

Liwei Yang, Juho Kim, Long Chen, Wei Wei, Jun Wang

Abstract read
In one paragraph

Article in Analytical chemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Liwei YangMultiplex Biotechnology Laboratory, Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York 11794, United States.ORCID 0000-0001-7834-0249
Juho KimInstitute for Systems Biology, Seattle, Washington 98109, United States.
Long ChenMultiplex Biotechnology Laboratory, Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York 11794, United States.
Wei WeiInstitute for Systems Biology, Seattle, Washington 98109, United States.ORCID 0000-0002-1018-7708
Jun WangMultiplex Biotechnology Laboratory, Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York 11794, United States.ORCID 0000-0002-8781-8248

Funding

Liquid biopsy-based toolkits for neoantigen and cognate TCR discovery for cancer immunotherapyR33CA256112 · NCI · INSTITUTE FOR SYSTEMS BIOLOGY · PI WEI, WEI · 2021 to 2023
$1.3M
Advanced Single-Cell Protein Analysis with Multiplex in Situ Tagging Array TechnologyR35GM151972 · NIGMS · STATE UNIVERSITY NEW YORK STONY BROOK · PI Jun Wang · 2024 to 2026
$1.2M
PRODIGE: A high-throughput tool for joint profiling of protein-DNA interactions and gene expression in single cellsR21GM140391 · NIGMS · INSTITUTE FOR SYSTEMS BIOLOGY · PI WEI, WEI · 2021 to 2022
$503k
High-Resolution Spatial MIST Technology for Functional Proteomic Study of Neuroinflammation in Alzheimer's DiseaseR21AG075087 · NIA · STATE UNIVERSITY NEW YORK STONY BROOK · PI WANG, JUN · 2022 to 2022
$463k
Single-cell Cyclic Multiplex in Situ Tagging to Advance Kidney ResearchR21DK138409 · NIDDK · STATE UNIVERSITY NEW YORK STONY BROOK · PI MALLIPATTU, SANDEEP K, WANG, JUN · 2023 to 2023
$337k
NCI NIH HHS R33 CA256112NIA NIH HHS R21 AG075087NIDDK NIH HHS R21 DK138409NIGMS NIH HHS R21 GM140391NIGMS NIH HHS R35 GM151972
6 · The paper itself

Abstract

The identification and characterization of immune cell subpopulations are critical to reveal cell development throughout life and immune responses to environmental factors. Next-generation sequencing technologies have dramatically advanced single-cell genomics and transcriptomics for immune cell classification. However, gene expression is often not correlated with protein expression, and immunotyping is mostly accepted in protein format. Current single-cell proteomic technologies are either limited in multiplex capacity or not sensitive enough to detect the critical functional proteins. Herein, we present a single-cell cyclic multiplex

Indexed as

BiomarkersProteomicsSingle-Cell AnalysisCell DifferentiationHumansLeukocytes, MononuclearT-LymphocytesBiomarkers

Identifiers

PMID39422499
PMCPMC11648578

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.