Evidence map›Paper›PMID 39421767›Full record

ArticleBiomedical optics express2024

FLUID: a fluorescence-friendly lipid-compatible ultrafast clearing method.

Zizheng Wang, Xiao Xiao, Ziwen Zhou, Yunyin Chen, Tianqi Xia, Xiangyi Sheng, Yiping Han, Wei Gong, Ke Si

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Zizheng WangDepartment of Psychiatry of the First Affiliated Hospital, Zhejiang University School of Medicine, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310058, China.
Xiao XiaoDepartment of Psychiatry of the First Affiliated Hospital, Zhejiang University School of Medicine, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310058, China.
Ziwen ZhouDepartment of Psychiatry of the First Affiliated Hospital, Zhejiang University School of Medicine, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310058, China.
Yunyin ChenLiangzhu Laboratory, MOE Frontier Science Center for Brain Science and Brain-machine Integration, State Key Laboratory of Brain-machine Intelligence, Zhejiang University, 1369 West Wenyi Road, Hangzhou 311121, China.
Tianqi XiaLiangzhu Laboratory, MOE Frontier Science Center for Brain Science and Brain-machine Integration, State Key Laboratory of Brain-machine Intelligence, Zhejiang University, 1369 West Wenyi Road, Hangzhou 311121, China.
Xiangyi ShengNHC and CAMS Key Laboratory of Medical Neurobiology, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou 310058, China.
Yiping HanNHC and CAMS Key Laboratory of Medical Neurobiology, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou 310058, China.
Wei GongNanhu Brain-computer Interface Institute, Hangzhou 311100, China.
Ke SiDepartment of Psychiatry of the First Affiliated Hospital, Zhejiang University School of Medicine, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310058, China.ORCID https://orcid.org/0000-0001-8328-4325

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Many clearing methods achieve high transparency by removing lipid components from tissues, which damages microstructure and limits their application in lipid research. As for methods which preserve lipid, it is difficult to balance transparency, fluorescence preservation and clearing speed. In this study, we propose a rapid water-based clearing method that is fluorescence-friendly and preserves lipid components. FLUID allows for preservation of endogenous fluorescence over 60 days. It shows negligible tissue distortion and is compatible with various types of fluorescent labeling and tissue staining methods. High quality imaging of human brain tissue and compatibility with pathological staining demonstrated the potential of our method for three-dimensional (3D) biopsy and clinical pathological diagnosis.

Identifiers

PMID39421767
PMCPMC11482171

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.