Evidence map›Paper›PMID 39421628›Full record

ArticleSovremennye tekhnologii v meditsine2024

Biomineralization of Human Genomic DNA into ZIF-8, a Zeolite-Like Metal-Organic Framework.

I H Shaykhutdinov, P V Iliasov, L V Limareva, A S Sustretov, D A Kokorev, A V Sokolov

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Sovremennye tekhnologii v meditsine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

I H ShaykhutdinovResearcher, Laboratory of New Medical Materials and Technologies, Professional Center for Education and Research in Genetic and Laboratory Technologies; Assistant, Chemistry Department, Pharmacy Institute; Samara State Medical University, 89 Chapaevskaya St., Samara, 443099, Russia.
P V IliasovLeading Researcher, Laboratory of Non-Infectious Immunology, Professional Center for Education and Research in Genetic and Laboratory Technologies; Samara State Medical University, 89 Chapaevskaya St., Samara, 443099, Russia.
L V LimarevaAssociate Professor, Head of the Laboratory of Non-Infectious Immunology, Professional Center for Education and Research in Genetic and Laboratory Technologies; Samara State Medical University, 89 Chapaevskaya St., Samara, 443099, Russia.
A S SustretovHead of the Laboratory of Human Metagenomics, Professional Center for Education and Research in Genetic and Laboratory Technologies; Samara State Medical University, 89 Chapaevskaya St., Samara, 443099, Russia.
D A KokorevResearcher, Laboratory of New Medical Materials and Technologies, Professional Center for Education and Research in Genetic and Laboratory Technologies; Assistant, Chemistry Department, Pharmacy Institute; Samara State Medical University, 89 Chapaevskaya St., Samara, 443099, Russia.
A V SokolovHead of the Laboratory of New Medical Materials and Technologies, Professional Center for Education and Research in Genetic and Laboratory Technologies; Samara State Medical University, 89 Chapaevskaya St., Samara, 443099, Russia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Materials and Methods: We studied human genomic DNA isolated from lymphocytes of peripheral blood of healthy volunteers using Proba-NK kit (DNA-Technology LLC, Russia). Genomic DNA purity and concentration was estimated spectrophotometrically at 260/280 nm using Tecan Infinity 200 Pro plate reader (Tecan Instruments, Austria). ZIF-8 was synthesized in the physiological conditions (37°C) by mixing zinc salt and 2-methylimidazole aqueous solutions at different molar ratios. Human genomic DNA was encapsulated into ZIF-8 in similar conditions. The obtained MOF and DNA@ZIF-8 composite were studied using X-ray powder diffraction at the Phaser D2 XRPD device (Bruker, USA), and the specific surface area was estimated using Autosorb iQ porosimetry analyzer (Quantachrome, USA). The encapsulated DNA was quantified by dissolving DNA@ZIF-8 composite in the citrate buffer. DNA integrity was assessed by real-time allele-specific PCR (AS-PCR) using the kits for single nucleotide polymorphisms (Lytech, Russia) at the Quantstudio 6 Pro PCR machine (Thermo Scientific, USA). In case of using the kits with electrophoretic detection, the amplification was performed on the thermal cycler T100 (Thermo Scientific, USA). Results: The polymer ZIF-8 and DNA@ZIF-8 composite were obtained at different molar ratios of zinc ions and 2-methylimidazole. We characterized their structure and specific surface area. Genomic DNA biomineralization efficacy was found to be about 7-8%. PCR indicated the integrity of non-selectively chosen loci within the biomineralized DNA. Conclusion: The study confirmed the possibility of human genomic DNA encapsulation into ZIF-8 metal-organic framework. After the biomineralization, DNA was found to preserve feasibility to be used in studies to investigate genetic constructs.

Indexed as

DNAMetal-Organic FrameworksZeolitesBiomineralizationGenome, HumanHumansImidazoles2-methylimidazoleDNAImidazolesMetal-Organic FrameworksZeolitesZIF-8 metal-organic frameworkbiomineralizationencapsulationgenetic vectorshuman genomic DNAmetal-organic frameworkZIF-8

Identifiers

PMID39421628
PMCPMC11482095

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.