Effect control of novel Hirudinaria manillensis extract on the molecular regulation of EGFR gene and its protein expression in HeLa cells.

P Zeebul Trinita Shannan, Susan G Suganya, M Ramesh, E Angel Jemima

Abstract read

In one paragraph

Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Receptor Binding, Functional Activity, and Cell Viability Assessment of Novel Marine-Based Hybrid Peptides fromMarine drugs · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

P Zeebul Trinita ShannanPG and Research Department of Zoology, Bishop Heber College, Affiliated to Bharathidasan University, Tiruchirappalli, Tamilnadu, 620017, India.

Susan G SuganyaPG and Research Department of Zoology, Bishop Heber College, Affiliated to Bharathidasan University, Tiruchirappalli, Tamilnadu, 620017, India. susangsuganya@gmail.com.

M RameshDepartment of Zoology, Thanthai Periyar Government Arts and Science College, Affiliated to Bharathidasan University, Tiruchirappalli, Tamilnadu, 620023, India.

E Angel JemimaDepartment of Biotechnology, Trichy Research Institute of Biotechnology, Trichy, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMost anticancer drugs possess the capacity to control precise molecular processes and gene-regulated protein expressions, which could enhance the efficacy of cancer treatments. Hirudinaria manillensis is prevalent in South Asia and has been employed for several decades in medical conditions based on its curative benefits. METHODS AND

resultsThe present study aimed to explore the molecular regulation of the target EGFR gene, and the protein expression of EGFR on the HeLa cell line. To achieve our aim, quantitative real-time PCR and immunocytochemistry assays were conducted. Interestingly, qRT-PCR results confirmed the downregulation of the EGFR gene on the HeLa cells in comparison with the β- actin internal control gene. Furthermore, immunocytochemistry assay results confirmed the decreasing level of EGFR gene expression in the HeLa cells.

conclusionTherefore, Hirudinaria manillensis could be a promising anticancer candidate for cervical cancer treatment.

Indexed as

ErbB ReceptorsGene Expression Regulation, NeoplasticHeLa CellsHumansEGFR protein, humanErbB ReceptorsEGFRHeLaImmunocytochemistryqRT-PCR

Identifiers

PMID39419922

DOI10.1007/s11033-024-10016-7

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.