Evidence map›Paper›PMID 39416778›Full record

ArticleFrontiers in immunology2024

ELISA-R: an R-based method for robust ELISA data analysis.

Taru S Dutt, John S Spencer, Burton R Karger, Amy Fox, Andres Obregon-Henao, Brendan K Podell, G Brooke Anderson, Marcela Henao-Tamayo

Abstract read
In one paragraph

Article in Frontiers in immunology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
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  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Taru S DuttDepartment of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, United States.
John S SpencerDepartment of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, United States.
Burton R KargerCollege of Osteopathic Medicine, University of New England, Biddeford, ME, United States.
Amy FoxDepartment of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, United States.
Andres Obregon-HenaoDepartment of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, United States.
Brendan K PodellDepartment of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, United States.
G Brooke AndersonEnvironmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO, United States.
Marcela Henao-TamayoDepartment of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, United States.

Funding

IMMUNE MECHANISMS OF PROTECTION AGAINST MYCOBACTERIUM TUBERCULOSIS CENTER (IMPAC-TB)75N93021C00029 · NIAID · SEATTLE CHILDREN'S HOSPITAL · PI COLER, RHEA · 2022 to 2025
$35.2M
Vaccine induced memory immunity in tuberculosisR01AI127475 · NIAID · COLORADO STATE UNIVERSITY · PI HENAO-TAMAYO, MARCELA I · 2017 to 2020
$1.5M
Non-tuberculous mycobacterium and B cells in the stimulation of ectopic germinal centers and immunological control of pulmonary tuberculosisR00AI173401 · NIAID · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI Taru S. Dutt · 2025 to 2026
$497k
Novel vaccine boosting candidates for tuberculosisR21AI121099 · NIAID · COLORADO STATE UNIVERSITY · PI HENAO-TAMAYO, MARCELA I · 2016 to 2017
$416k
Non-tuberculous mycobacterium and B cells in the stimulation of ectopic germinal centers and immunological control of pulmonary tuberculosisK99AI173401 · NIAID · COLORADO STATE UNIVERSITY · PI DUTT, TARU S. · 2023 to 2024
$225k
NIAID NIH HHS 75N93021C00029NIAID NIH HHS K99 AI173401NIAID NIH HHS R00 AI173401NIAID NIH HHS R01 AI127475NIAID NIH HHS R21 AI121099
6 · The paper itself

Abstract

Enzyme-linked immunosorbent assay (ELISA) is a technique to detect the presence of an antigen or antibody in a sample. ELISA is a simple and cost-effective method that has been used for evaluating vaccine efficacy by detecting the presence of antibodies against viral/bacterial antigens and diagnosis of disease stages. Traditional ELISA data analysis utilizes a standard curve of known analyte, and the concentration of the unknown sample is determined by comparing its observed optical density against the standard curve. However, in the case of vaccine research for complicated bacteria such as

Indexed as

Enzyme-Linked Immunosorbent AssayMycobacterium tuberculosisAnimalsAntibodies, BacterialAntigens, BacterialData AnalysisMiceTuberculosisAntibodies, BacterialAntigens, Bacterialantibodiescurve-fittingdata analysisELISAendpoint titerMycobacterium lepraeMycobacterium tuberculosis

Identifiers

PMID39416778
PMCPMC11479990

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.