Evidence map›Paper›PMID 39416011›Full record

ArticlebioRxiv : the preprint server for biology2025

Suspended Tissue Open Microfluidic Patterning (STOMP).

Amanda J Haack, Lauren G Brown, Alex J Goldstein, Priti Mulimani, Jean Berthier, Asha R Viswanathan, Irina Kopyeva, Jamison M Whitten, Ariel Lin, Serena H Nguyen and 13 more

Abstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

23 authors.

Amanda J HaackDepartment of Chemistry, University of Washington, Seattle, WA, 98195 USA.ORCID 0000-0002-0066-7556
Lauren G BrownDepartment of Chemistry, University of Washington, Seattle, WA, 98195 USA.ORCID 0000-0002-5932-2691
Alex J GoldsteinInstitute for Stem Cell & Regenerative Medicine, University of Washington, Seattle, WA, 98109 USA.
Priti MulimaniInstitute for Stem Cell & Regenerative Medicine, University of Washington, Seattle, WA, 98109 USA.ORCID 0000-0001-7488-0192
Jean BerthierDepartment of Chemistry, University of Washington, Seattle, WA, 98195 USA.
Asha R ViswanathanDepartment of Chemistry, University of Washington, Seattle, WA, 98195 USA.ORCID 0009-0009-2363-2311
Irina KopyevaDepartment of Bioengineering, University of Washington, Seattle, WA, 98195 USA.ORCID 0000-0003-3740-6971
Jamison M WhittenDepartment of Chemistry, University of Washington, Seattle, WA, 98195 USA.ORCID 0009-0009-7475-7771
Ariel LinDepartment of Chemistry, University of Washington, Seattle, WA, 98195 USA.ORCID 0009-0000-0443-3987
Serena H NguyenDepartment of Chemistry, University of Washington, Seattle, WA, 98195 USA.ORCID 0009-0000-2132-6518
Thomas P LeahyInstitute for Stem Cell & Regenerative Medicine, University of Washington, Seattle, WA, 98109 USA.ORCID 0000-0002-2749-6021
Ella E BoukerDepartment of Chemistry, University of Washington, Seattle, WA, 98195 USA.ORCID 0009-0009-9855-8646
Ruby M PadgettInstitute for Stem Cell & Regenerative Medicine, University of Washington, Seattle, WA, 98109 USA.ORCID 0000-0002-9693-446X
Natalie A MazzawiInstitute for Stem Cell & Regenerative Medicine, University of Washington, Seattle, WA, 98109 USA.
Jodie C TokihiroDepartment of Chemistry, University of Washington, Seattle, WA, 98195 USA.ORCID 0000-0003-0958-2249
Ross C BrethertonInstitute for Stem Cell & Regenerative Medicine, University of Washington, Seattle, WA, 98109 USA.ORCID 0000-0002-9567-8556
Aaliyah WuDepartment of Chemistry, University of Washington, Seattle, WA, 98195 USA.
Stephen J TapscottHuman Biology Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA.ORCID 0000-0002-0319-0968
Cole A DeForestDepartment of Chemistry, University of Washington, Seattle, WA, 98195 USA.ORCID 0000-0003-0337-3577
Tracy E PopowicsInstitute for Stem Cell & Regenerative Medicine, University of Washington, Seattle, WA, 98109 USA.ORCID 0000-0003-2981-0309
Erwin BerthierDepartment of Chemistry, University of Washington, Seattle, WA, 98195 USA.ORCID 0000-0002-4421-9034
Nathan J SniadeckiInstitute for Stem Cell & Regenerative Medicine, University of Washington, Seattle, WA, 98109 USA.ORCID 0000-0001-6960-4492
Ashleigh B ThebergeDepartment of Chemistry, University of Washington, Seattle, WA, 98195 USA.ORCID 0000-0002-3394-1435

Funding

XENOBIOTIC BIOTRANSFORMATION AND DISPOSITIONP30ES007033 · NIEHS · UNIVERSITY OF WASHINGTON · PI Nicole Ann Errett · 1995 to 2026
$42.5M
Wellstone Muscular Dystrophy Specialized Research Center (Seattle)P50AR065139 · NIAMS · UNIVERSITY OF WASHINGTON · PI MICHAEL REGNIER · 2018 to 2026
$15.9M
Interdisciplinary Tranining in Cancer ResearchT32CA080416 · NCI · UNIVERSITY OF WASHINGTON · PI STODDARD, BARRY L. · 1998 to 2023
$9.7M
NRSA Training CoreTL1TR002318 · NCATS · UNIVERSITY OF WASHINGTON · PI Megan Moore · 2017 to 2026
$8.4M
Theberge Admin Supp Undergraduate Summer Research ExperiencesR35GM128648 · NIGMS · UNIVERSITY OF WASHINGTON · PI Ashleigh Brooks Theberge · 2018 to 2026
$4.7M
Mimicking, Exploiting, and Understanding Biology's Heterogeneity in 4DR35GM138036 · NIGMS · UNIVERSITY OF WASHINGTON · PI Cole A DeForest · 2020 to 2026
$2.3M
Mechanotransduction by Melusin in Cardiac HypertrophyR01HL149734 · NHLBI · UNIVERSITY OF WASHINGTON · PI SNIADECKI, NATHAN JOHN · 2020 to 2023
$2.1M
Comprehensive Training in Inter-Disciplinary Oral Health ResearchR90DE023059 · NIDCR · UNIVERSITY OF WASHINGTON · PI Robert Aaron Cornell · 2012 to 2026
$1.2M
Engineered 3D Periodontal Tissue Constructs for Defining Functional Outcomes of Regenerative ProcessesR03DE029827 · NIDCR · UNIVERSITY OF WASHINGTON · PI POPOWICS, TRACY E · 2020 to 2021
$353k
Generating multilayered engineered heart tissue patches to mimic physiological thickness and function using open microfluidicsF30HL158030 · NHLBI · UNIVERSITY OF WASHINGTON · PI HAACK, AMANDA JEAN · 2021 to 2025
$192k
NCATS NIH HHS TL1 TR002318NCI NIH HHS T32 CA080416NHLBI NIH HHS F30 HL158030NHLBI NIH HHS R01 HL149734NIAMS NIH HHS P50 AR065139NIDCR NIH HHS R03 DE029827NIDCR NIH HHS R90 DE023059NIEHS NIH HHS P30 ES007033NIGMS NIH HHS R35 GM128648NIGMS NIH HHS R35 GM138036
6 · The paper itself

Abstract

Free-standing tissue structures tethered between pillars are powerful mechanobiology tools for studying cell contraction. To model interfaces ubiquitous in natural tissues and upgrade existing single-region suspended constructs, we developed Suspended Tissue Open Microfluidic Patterning (STOMP), a method to create multiregional suspended tissues. STOMP uses open microfluidics and capillary pinning to pattern subregions within free-standing tissues, facilitating the study of complex tissue interfaces, such as diseased-healthy boundaries (e.g., fibrotic-healthy) and tissue-type interfaces (e.g., bone-ligament). We observed altered contractile dynamics in fibrotic-healthy engineered heart tissues compared to single-region tissues and differing contractility in bone-ligament enthesis constructs compared to single-tissue periodontal ligament models. STOMP is a versatile platform - surface tension-driven patterning removes material requirements common with other patterning methods (e.g., shear-thinning, photopolymerizable) allowing tissue generation in multiple geometries with native extracellular matrices and advanced 4D materials. STOMP combines the contractile functionality of suspended tissues with precise patterning, enabling dynamic and spatially controlled studies.

Identifiers

PMID39416011
PMCPMC11482760

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.