Evidence map›Paper›PMID 39411944›Full record

ArticleCombinatorial chemistry & high throughput screening2025

KLF15 Transcription Activated Tachykinin Precursor 1 to Induce Perineural Invasion in Head and Neck Squamous Cell Carcinoma.

Yupeng Shen, Yong Shi, Xin Liu, Ming Zhang, Liang Zhou, Chengzhi Xu

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Article in Combinatorial chemistry & high throughput screening, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Yupeng ShenDepartment of Otolaryngology-Head and Neck Surgery, The first hospital of Hebei Medical University, Shijiazhuang, 050000, China.
Yong ShiENT Institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China.
Xin LiuDepartment of Otolaryngology-Head and Neck Surgery, The first hospital of Hebei Medical University, Shijiazhuang, 050000, China.
Ming ZhangENT Institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China.
Liang ZhouENT Institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China.
Chengzhi XuENT Institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China.

Funding

Key Science and Technology Research Program of Hebei Provincial Health Commission 20190183National Natural Science Foundation of China 82372972
6 · The paper itself

Abstract

objectiveIn this study, we aimed to identify novel biomarkers related to Peripheral Neural Invasion (PNI) in head and neck squamous cell carcinoma (HNSCC).

methodsThe PNI-related differentially expressed mRNAs (DE-mRNAs) in HNSCC were identified to construct a PNI-related risk score model. The expression level and ROC curve for Tachykinin Precursor 1 (TAC1) were calculated. Additionally, two kinds of in vitro models of PNI were established for investigation, including the Matrigel-PNI model and the Transwell-PNI model. Furthermore, the transcription factor of the TAC1 was predicted and verified by qRTPCR.

resultsA total of 139 DE-mRNAs were identified in PNI positive and negative groups of HNSCC patients. The risk-score marker model incorporating 20 PNI-related DE-mRNAs was established. The TAC1 was identified as a potential highly expressed PNI marker, which exhibited good performance in predicting PNI events. Patients with higher TAC1 expressions demonstrated significantly shorter survival rates compared to those with lower TAC1 expressions in HNSCC. Besides, the knockdown of TAC1 significantly repressed neural invasion in HNSCC cells in vitro, according to the Matrigel-PNI model and Transwell-PNI model. Furthermore, KLF15 was predicted and verified as a transcription activator of TAC1 in HNSCC.

conclusionThis study highlights that the activation of KLF15 transcription of TAC1 promotes PNI in HNSCC cells, which provides guidance regarding the molecular diagnosis of PNI in HNSCC cells.

Indexed as

Biomarkers, TumorGene Expression Regulation, NeoplasticHead and Neck NeoplasmsKruppel-Like Transcription FactorsPerineuronal NetsSquamous Cell Carcinoma of Head and NeckTachykininsTranscriptional ActivationCell Line, TumorGene Knockdown TechniquesHumansNeoplasm InvasivenessRNA, MessengerBiomarkers, TumorKLF15 protein, humanKruppel-Like Transcription FactorsRNA, MessengerTAC1 protein, humanTachykininsbiomarkerHead and neck squamous cell carcinoma (HNSCC)KLF15LASSO analysis.peripheral neural invasion (PNI)TAC1

Identifiers

PMID39411944

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.