Evidence map›Paper›PMID 39408610›Full record

ArticleInternational journal of molecular sciences2024

Assessing Biomarkers of Porcine Kidneys under Normothermic Machine Perfusion-Can We Gain Insight into a Marginal Organ?

Carla Steinhauser, Abdulbaki Yakac, Wenke Markgraf, Susanne Kromnik, Andreas Döcke, Philipp Talhofer, Christine Thiele, Hagen Malberg, Ulrich Sommer, Gustavo B Baretton and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Carla SteinhauserDepartment of Urology, Faculty of Medicine, University Hospital Carl Gustav Carus, Technische Universität Dresden, D-01309 Dresden, Germany.ORCID 0000-0002-9011-0822
Abdulbaki YakacDepartment of Urology, Faculty of Medicine, University Hospital Carl Gustav Carus, Technische Universität Dresden, D-01309 Dresden, Germany.
Wenke MarkgrafInstitute of Biomedical Engineering, Technische Universität Dresden, D-01309 Dresden, Germany.ORCID 0000-0003-0090-8265
Susanne KromnikInstitute of Biomedical Engineering, Technische Universität Dresden, D-01309 Dresden, Germany.
Andreas DöckeInstitute of Biomedical Engineering, Technische Universität Dresden, D-01309 Dresden, Germany.ORCID 0009-0000-8786-5338
Philipp TalhoferInstitute of Biomedical Engineering, Technische Universität Dresden, D-01309 Dresden, Germany.
Christine ThieleInstitute of Biomedical Engineering, Technische Universität Dresden, D-01309 Dresden, Germany.
Hagen MalbergInstitute of Biomedical Engineering, Technische Universität Dresden, D-01309 Dresden, Germany.
Ulrich SommerInstitute of Pathology, Faculty of Medicine, University Hospital Carl Gustav Carus, Technische Universität Dresden, D-01309 Dresden, Germany.
Gustavo B BarettonInstitute of Pathology, Faculty of Medicine, University Hospital Carl Gustav Carus, Technische Universität Dresden, D-01309 Dresden, Germany.
Susanne FüsselDepartment of Urology, Faculty of Medicine, University Hospital Carl Gustav Carus, Technische Universität Dresden, D-01309 Dresden, Germany.ORCID 0000-0002-5339-9460
Christian ThomasDepartment of Urology, Faculty of Medicine, University Hospital Carl Gustav Carus, Technische Universität Dresden, D-01309 Dresden, Germany.
Juliane PutzDepartment of Urology, Faculty of Medicine, University Hospital Carl Gustav Carus, Technische Universität Dresden, D-01309 Dresden, Germany.

Funding

European Regional Development Fund 100382963
6 · The paper itself

Abstract

To identify potentially transplantable organs in a pool of marginal kidneys, 33 porcine slaughterhouse kidneys were perfused for 4 h with whole blood. During the normothermic perfusion, plasma, urine, and tissue samples were taken. Several biomarkers for tubule injury, endothelial activation, and inflammatory response were evaluated for a potential correlation with macroscopic appearance, histology, and filtration activity. Generally, biomarker levels increased during perfusion. TLR-4, EDN-1, and NGAL were not associated with any classification. In contrast, a steeper increase in NAG and IL-6 in plasma correlated with a poor macroscopic appearance at 4 h, indicating a higher inflammatory response in the kidneys with worse macroscopy early on, potentially due to more damage at the tubules. Although long-term effects on the graft could not be assessed in this setting, early observation under machine perfusion with whole blood was feasible. It allowed the assessment of kidneys under conditions comparable to reperfusion. This setting could give surgeons further insight into the quality of marginal kidneys and an opportunity to pre-treat them.

Indexed as

BiomarkersKidneyPerfusionAnimalsKidney TransplantationOrgan PreservationSwineBiomarkersbiomarkerkidneynormothermic machine perfusionpigreperfusion-injury

Identifiers

PMID39408610
PMCPMC11476884

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.