Article in Molecules (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registry
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what money
Authors and funding
13 authors.
Matheus Cavalcanti de BarrosDepartamento de Bioquímica, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil.ORCID 0000-0002-8769-0221
Ana Patrícia Silva de OliveiraDepartamento de Bioquímica, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil.ORCID 0000-0003-2076-0229
Franciane Gonçalves Dos SantosDepartamento de Química Fundamental, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil.
Fabiana Aparecida Cavalcante SilvaCentro de Tecnologias Estratégicas do Nordeste, Recife 50740-545, PE, Brazil.ORCID 0000-0002-4674-895X
Thais Meira MenezesDepartamento de Química Fundamental, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil.
Gustavo de Miranda SeabraDepartment of Medicinal Chemistry, Center for Natural Products, Drug Discovery and Development, College of Pharmacy, University of Florida, Gainseville, FL 32611, USA.ORCID 0000-0002-1303-4483
Juliana Sakamoto YonedaLaboratório Nacional de Luz Síncrotron, Centro Nacional de Pesquisa em Energia e Materiais, Campinas 13083-100, SP, Brazil.ORCID 0000-0003-2814-3022
Luana Cassandra Breitenbach Barroso CoelhoDepartamento de Bioquímica, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil.ORCID 0000-0002-1013-0023
Maria Lígia Rodrigues MacedoDepartamento de Tecnologia de Alimentos e da Saúde, Faculdade de Ciências Farmacêuticas, Alimentos e 22 Nutrição, Universidade Federal do Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil.
Thiago Henrique NapoleãoDepartamento de Bioquímica, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil.ORCID 0000-0002-0065-2602
Thâmarah de Albuquerque LimaDepartamento de Bioquímica, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil.
Jorge Luiz NevesDepartamento de Química Fundamental, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil.
Patrícia Maria Guedes PaivaDepartamento de Bioquímica, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil.
Funding
Conselho Nacional de Desenvolvimento Científico e Tecnológico 407192/2018-2Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Finance Code 001Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco APQ-0108-2.08/14Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco APQ-1491-2.08/22
6 · The paper itself
Abstract
This study characterized the binding mechanisms of the lectin cMoL (from Moringa oleifera seeds) to carbohydrates using spectroscopy and molecular dynamics (MD). The interaction with carbohydrates was studied by evaluating lectin fluorescence emission after titration with glucose or galactose (2.0-11 mM). The Stern-Volmer constant (Ksv), binding constant (Ka), Gibbs free energy (∆G), and Hill coefficient were calculated. After the urea-induced denaturation of cMoL, evaluations were performed using fluorescence spectroscopy, circular dichroism (CD), and hemagglutinating activity (HA) evaluations. The MD simulations were performed using the Amber 20 package. The decrease in Ksv revealed that cMoL interacts with carbohydrates via a static mechanism. The cMoL bound carbohydrates spontaneously (ΔG < 0) and presented a Ka on the order of 10
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.
Carbohydrate-Binding Mechanism of the Coagulant Lectin from · full record | OpenQuestion